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- W2167000255 abstract "A method is described for studying the coupling ratio of the Na+/K+ pump, i.e., the ratio of pump-mediated fluxes of Na+ and K+, in a reconstituted system. The method is based on the comparison of the pump-generated current with the rate of K+ transport. Na+/K+-ATPase from kidney is incorporated into the membrane of artificial lipid vesicles; ATPase molecules with outward-oriented ATP-binding site are activated by addition of ATP to the medium. Using oxonol VI as a potential-sensitive dye for measuring transmembrane voltage, the pump current is determined from the change of voltage with time t. In a second set of experiments, the membrane is made selectively K+-permeable by addition of valinomycin, so that the membrane voltage U is equal to the Nernst potential of K+. Under this condition, dU/dt reflects the change of intravesicular K+ concentration and thus the flux of K+. Values of the Na+/K+ coupling ratio determined in this way are close to 1.5 in the experimental range (10-75 mM) of extravesicular (cytoplasmic) Na+ concentrations." @default.
- W2167000255 created "2016-06-24" @default.
- W2167000255 creator A5001565953 @default.
- W2167000255 creator A5006104880 @default.
- W2167000255 creator A5038060167 @default.
- W2167000255 date "1989-06-01" @default.
- W2167000255 modified "2023-10-06" @default.
- W2167000255 title "Pump current and Na+/K+ coupling ratio of Na+/K+-ATPase in reconstituted lipid vesicles" @default.
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- W2167000255 doi "https://doi.org/10.1016/0005-2736(89)90044-8" @default.
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