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• W2208051888 abstract "In electron tomography, accurate alignment of tilt series is an essential step in attaining high-resolution 3D reconstructions. Nevertheless, quantitative assessment of alignment quality has remained a challenging issue, even though many alignment methods have been reported. Here, we report a fast and accurate method, tomoAlignEval, based on the Beer–Lambert law, for the evaluation of alignment quality. Our method is able to globally estimate the alignment accuracy by measuring the goodness of log-linear relationship of the beam intensity attenuations at different tilt angles. Extensive tests with experimental data demonstrated its robust performance with stained and cryo samples. Our method is not only significantly faster but also more sensitive than measurements of tomogram resolution using Fourier shell correlation method (FSCe/o). From these tests, we also conclude that while current alignment methods are sufficiently accurate for stained samples, inaccurate alignments remain a major limitation for high resolution cryo-electron tomography." @default.
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• W2208051888 date "2015-11-01" @default.
• W2208051888 modified "2023-09-24" @default.
• W2208051888 title "A fast cross-validation method for alignment of electron tomography images based on Beer–Lambert law" @default.
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• W2208051888 doi "https://doi.org/10.1016/j.jsb.2015.10.004" @default.
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