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• W2220382232 endingPage "C509" @default.
• W2220382232 startingPage "C501" @default.
• W2220382232 abstract "O2 plays a pivotal role in aerobic metabolism and regulation of cell and tissue function. Local differences and fluctuations in tissue O2 levels are well documented; however, the physiological significance of O2 microgradients, particularly at the subcellular level, remains poorly understood. Using the cell-penetrating phosphorescent O2 probe Pt-Glc and confocal fluorescence microscopy, we visualized O2 distribution in individual giant (>100-μm) umbrella cells located superficially in the urinary bladder epithelium. We optimized conditions for in vivo phosphorescent staining of the inner surface of the mouse bladder and subsequent ex vivo analysis of excised live tissue. Imaging experiments revealed significant (≤85 μM) and heterogeneous deoxygenation within respiring umbrella cells, with radial O2 gradients of up to 40 μM across the cell, or ∼0.6 μM/μm. Deeply deoxygenated (5-15 μM O2) regions were seen to correspond to the areas enriched with polarized mitochondria. Pharmacological activation of mitochondrial respiration decreased oxygenation and O2 gradients in umbrella cells, while inhibition with antimycin A dissipated the gradients and caused gradual reoxygenation of the tissue to ambient levels. Detailed three-dimensional maps of O2 distribution potentially can be used for the modeling of intracellular O2-dependent enzymatic reactions and downstream processes, such as hypoxia-inducible factor signaling. Further ex vivo and in vivo studies on intracellular and tissue O2 gradients using confocal imaging can shed light on the molecular mechanisms regulating O2-dependent (patho)physiological processes in the bladder and other tissues." @default.
• W2220382232 created "2016-06-24" @default.
• W2220382232 creator A5024611651 @default.
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• W2220382232 creator A5063081039 @default.
• W2220382232 creator A5068632457 @default.
• W2220382232 creator A5084928512 @default.
• W2220382232 date "2015-10-01" @default.
• W2220382232 modified "2023-09-26" @default.
• W2220382232 title "Imaging of oxygen gradients in giant umbrella cells: an ex vivo PLIM study" @default.
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• W2220382232 doi "https://doi.org/10.1152/ajpcell.00121.2015" @default.
• W2220382232 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26246428" @default.
• W2220382232 hasPublicationYear "2015" @default.
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