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• W2280211694 abstract "Induced pluripotent stem cell-derived cardiomyocytes (IPS-CM) are considered by many to be the cornerstone of future approaches to repair the diseased heart. However, current methods for producing IPS-CM typically yield highly variable populations with low batch-to-batch reproducibility. The underlying reasons for this are not fully understood. Here we report on a systematized approach to investigate the effect of maturation in embryoid bodies (EB) vs. on plate culture on spontaneous activity and regional Ca(2+) synchronization in IPS-CM clusters. A detailed analysis of the temporal and spatial organization of Ca(2+) spikes in IPS-CM clusters revealed that the disaggregation of EBs between 0.5 and 2 weeks produced IPS-CM characterized by spontaneous beating and high levels of regional Ca(2+) synchronization. These phenomena were typically absent in IPS-CM obtained from older EBs (>2 weeks). The maintenance of all spontaneously active IPS-CM clusters under on plate culture conditions promoted the progressive reduction in regional Ca(2+) synchronization and the loss of spontaneous Ca(2+) spiking. Raising the extracellular [Ca(2+)] surrounding these quiescent IPS-CM clusters from ~0.4 to 1.8 mM unmasked discrete behaviors typified by either (a) long-lasting Ca(2+) elevation that returned to baseline or (b) persistent, large-amplitude Ca(2+) oscillations around an increased cytoplasmic [Ca(2+)]. The different responses of IPS-CM to elevated extracellular [Ca(2+)] could be traced back to their routes of derivation. The data point to the possibility of predictably influencing IPS-CM phenotype and response to external activation via defined interventions at early stages in their maturation." @default.
• W2280211694 created "2016-06-24" @default.
• W2280211694 creator A5011265697 @default.
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• W2280211694 creator A5066576644 @default.
• W2280211694 date "2016-01-13" @default.
• W2280211694 modified "2023-10-18" @default.
• W2280211694 title "A Systemized Approach to Investigate Ca2+ Synchronization in Clusters of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes" @default.
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• W2280211694 doi "https://doi.org/10.3389/fcell.2015.00089" @default.
• W2280211694 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4710702" @default.
• W2280211694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26793710" @default.
• W2280211694 hasPublicationYear "2016" @default.
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