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- W2308995155 abstract "This experiment designed to construct,express and purify a recombinant abrin B chain protein(r ATB),and then assess its antigenicity and toxicity.r ATB was prepared by replacing rare codon with synonymouscodons of high-frequency and subcloned to construct recombinant expression vector p QE80L-ATB.Afterexpression and purification,r ATB was determined by immunoblotting and MTS for its antigenicity and toxicity.Datashowed that an 804 bp gene encoding the recombinant protein with 268 amino acid residues was acquired.M15/p QE80L-ATB was induced at 30 ℃ by 0.1 mmol/L IPTG for 5 h,and the relative mass of ATB was approximate30 k Da.SDS-PAGE indicated that the purity of the protein was up to 97% after Ni-NTA purification;Western blotindicated that r ATB could react specifically with the rabbit p Ab against AT;MTS showed that r ATB was non-toxiceven at high doses.In conclusion,r ATB from E.coli possess good antigenicity and can be highly expressed,whichproved a base for developing effective and non-toxic vaccine." @default.
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- W2308995155 date "2016-01-01" @default.
- W2308995155 modified "2023-09-23" @default.
- W2308995155 title "Construction and characterization of a recombinant abrin B chain protein" @default.
- W2308995155 hasPublicationYear "2016" @default.
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