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- W2313586587 abstract "This study was done to investigate the effects of modified nucleotide sequences around the initiation codon on gene expression efficiency in Escherichia coli. A human tumor necrosis factor-β-galactosidase fused gene was used to randomly modify both the upstream nucleotide sequence from the initiation codon (— 4 to — 1) and the silent third letter of the codons corresponding to the 4 amino acid residues of the N-terminal region. It was observed that the frequency of adenine nucleotide usage in this region was higher in the high expression group than in the low expression group." @default.
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- W2313586587 date "1988-01-01" @default.
- W2313586587 modified "2023-09-24" @default.
- W2313586587 title "Increased expression in Escherichia coli of human tumor necrosis factor through in Vitro mutagenesis around the initiation codon." @default.
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- W2313586587 doi "https://doi.org/10.1271/bbb1961.52.1331" @default.
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