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- W2344554219 abstract "The cyclohexanol dehydrogenase (ChnA), produced by Rhodococcus sp. TK6, which is capable of growth on cyclohexanol as the sole carbon source, has been previously purified and characterized. However, the current study cloned the complete gene (chnA) for ChnA and its flanking regions using a combination of a polymerase chain reaction (PCR) based on the N-terminal amino acid sequence of the purified ChnA and plaque hybridization from a phage library of Rhodococcus sp. TK6. A sequence analysis of the 5,965-bp DNA fragment revealed five potential open reading frames (ORFs) designated as partial pte (phosphotriesterase), acs (acyl-CoA synthetase), scd (short chain dehydrogenase), stp (sugar transporter), and chnA (cyclohexanol dehydrogenase), respectively. The deduced amino acid sequence of the chnA gene exhibited a similarity of up to 53% with members of the short-chain dehydrogenase/reductase(SDR) family. The chnA gene was expressed using the pET21a(+) system in Escherichia coli. The activity of the expressed ChnA was then confirmed (13.6 U/mg of protein) and its properties investigated." @default.
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- W2344554219 date "2005-12-01" @default.
- W2344554219 modified "2023-09-27" @default.
- W2344554219 title "Cloning and Characterization of Cyclohexanol Dehydrogenase Gene from Rhodococcus sp. TK6" @default.
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