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- W2351974551 abstract "A pair of primers for HBx gene was synthesized according to the sequences of HBV genome in GenBank. The DNA of HBV genome were isolated from a new HBV field strain from a patient with chronic viral infection and used as template for amplification HBx coding sequence. And then the amplified HBx coding sequence was cloned into the Bait vector pGBKT7. Yeast AH109 with the vector expressing HBx protein fused in BD peptide was identified through the phenotype and screened cellular targets that are associated with the viral protein. By screening a pretransformed human liver cDNA library using a Gal 4 yeast two-hybrid system, four cellular proteins were able to be identified as new HBx interacting partners such as Aldolase B, Complement component 8, alpha polypeptide, Hypothetical protein MGC13047 and Hepsin." @default.
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- W2351974551 date "2004-01-01" @default.
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- W2351974551 title "Screening and Identification of HBx-Associated Cellular Proteins" @default.
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