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- W2355517054 abstract "To establish a sensitive and specific high performance liquid chromatography( HPLC) method for simultaneous determination of flavone glycosides( Rutin,Quercetin,Kaempferol) in Chestnut involucre. The HPLC separation was performed on an Agilent Extend-C18column( 4. 6 mm × 250 mm,5 μm) and detected with an UV detector at the wavelength of 360 nm. Gradient elution was carried out with acetonitrile-phosphoric buffer( pH = 3) at the flow rate of 1. 0mL / min. The column temperature was set at 25 ℃. The calibration curves were linear in the range of 0. 067 ~ 0. 67μg /mL for rutin,0. 046 ~ 0. 46 μg / mL for quercetin and 0. 044 ~ 0. 44 μg / mL for kaempferol( r ≥ 0. 9990),respectively. The average recoveries of rutin,kaempferol and quercetin were 99. 60%( RSD = 3. 20%),99. 20%( RSD =3. 30%) and 97. 90%( RSD = 4. 00%),respectively. The content of rutin,quercetin,kaempferol were 5. 68,0. 62,1. 94 mg / g respectively. This method is simple,accurate,reliable and hence can be used as a method for the determination of flavone in Chestnut involucre." @default.
- W2355517054 created "2016-06-24" @default.
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- W2355517054 date "2013-01-01" @default.
- W2355517054 modified "2023-09-23" @default.
- W2355517054 title "Simultaneous Determination of Three Flavone Glycosides in Chestnut Involucre by HPLC" @default.
- W2355517054 hasPublicationYear "2013" @default.
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