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- W2362675809 abstract "This study was designed to explore the toxic effects of cadmium on piglet sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion,followed by differential plating and were identified by oil red O staining and immunocytochemical methods. Piglet sertoli cells were cultured and classified into five groups,i.e,control group without cadmium chloride; and 4 separate groups exposed to 10,20,40 and 80μmol·L-1 cadmium chloride. We found that highly purified sertoli cells were isolated by the two-step enzyme digestion and the sertoli cells' growth was inhibited by cadmium chloride at 10μmol·L-1,compared with the control group. The MDA content was increased but the SOD and GSH Px activity of the sertoli cells were decreased by exposure to cadmium chloride at 10μmol·L-1. Moreover,DNA damage of sertoli cells was observed by cadmium chloride at 10μmol·L-1 and the damage showed a significant dose-effect relation with the increasing CdCl2 concentration." @default.
- W2362675809 created "2016-06-24" @default.
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- W2362675809 date "2010-01-01" @default.
- W2362675809 modified "2023-09-24" @default.
- W2362675809 title "DNA damage and decrease of cellular antioxidase activity in piglet sertoli cells exposed to cadmium" @default.
- W2362675809 hasPublicationYear "2010" @default.
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