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- W2364503096 abstract "Objective: To investigate the method of the guinea-pig ventricular myocytes and setup the stage for patch-clamping technique. Method: Single myocytes were isolated enzymatically from the guinea-pig ventricle. It was recorded that the action potential of different basic circle length, ICa-L and IK of ventricular myocytes in W-C Mode. Results: The isolated myocytes were survived by 90% with enzymatic dissociation; the resting potential of single myocytes was -74.0±2.2mV, APD90 313.2±20.72 ms when BCL was 1 000 ms; the peak of ICa-L current density -7.36±1.10 pA/pF, -1.22±0.49 pA/pF after perfusing with 0.1 mmol/L verapamil respectively, and the inward current was eliminated with perfusion of 100 μmol/L CdCl2; IK was 4.88±0.96 pA/pF, 3.48±0.37 pA/pF after 2μmol/L Dofetilide respectively. Conclusion: The single ventricular myocytes isolated enzymatically can be applied for patch-clamping experiments successfully; the patch-clamping technique can service for advanced electrophysiological research." @default.
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- W2364503096 date "2007-01-01" @default.
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- W2364503096 title "Isolation of the ventricular myocytes and observation of its electrophysiological properties in guinea-pig" @default.
- W2364503096 hasPublicationYear "2007" @default.
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