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- W2367329650 abstract "Aim:To construct a medicine screening system targeting on HIV-1 promoter.Methods:The gene of HIV-1 promoter was inserted into the luciferase reporter vector pGL4.17,and the plasmid of pGL4.17-HIV-1P was transfected into H9 cells.The transfected cells were screened by G418.Four kinds of traditional Chinese medicine(Eucommia Bark,Baical Skullcap Root,Lightyellow Sophora Root,and Shuanghuanglian)decoction were given to rats intragastrically and the medicated serum were collected.After the stable transfected H9 cells were stimulated by the four kinds of medicated serum,luciferase activity of each groups were detected.Results:There were significant difference in luciferase activity among these groups(F=1 820.333,P0.001).The Shuanghuanglian could depress the luciferase activity remarkably(P0.05),Eucommia Bark,Baical Skullcap Root and Lightyellow Sophora Root could enhance it(P0.05).Conclusion:A medicine screening system based on HIV-1 promoter is established successfully and Shuanghuanglian may have the potential anti-HIV-1 effect." @default.
- W2367329650 created "2016-06-24" @default.
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- W2367329650 date "2011-01-01" @default.
- W2367329650 modified "2023-09-24" @default.
- W2367329650 title "Establishment of a screening assay based on HIV-1 promoter" @default.
- W2367329650 hasPublicationYear "2011" @default.
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