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- W2371970899 abstract "OBJECTIVE To investigate the difference of cytotoxic effects of hydroxycamptothecin( HCPT) on human lung cancer cells A549 and human embryo lung fibroblast cells MRC-5. METHODS A549 cells and MRC-5 cells were treated with HCPT 20-200 μmol·L- 1for 24,48 and 72 h,or pulse treated with HCPT 50-400 μmol·L- 1for 24 h along with 5 d release. Cell survival was detected by MTT assay. Morphological changes for both types of cells were observed under an inverted phase-contrast microscope. Cell cycle and apoptosis in both cells treated with HCPT 50 μmol·L- 1for 48 h were determined by flow cytometry. RESULTS HCPT 20- 200 μmol·L- 1inhibited the survival of both cells in a concentration-dependent manner and more cytotoxicity was observed in A549 cells for 48 h. The concentration-effect correlation coefficient( r) of HCPT in A549 and MRC-5 cells for 48 h was 0. 898( P = 0. 015) and 0. 996( P = 2. 56E- 5) respectively. The inhibition rates were significantly different between A549 and MRC-5 cells with treatment of HCPT 20,50,80,100, 160 and 200 μmol·L- 1for 48 h( P 0. 05). The IC50of HCPT on A549 and MRC-5 cells was( 24. 00 ± 0. 69) μmol·L- 1 and( 123. 63 ± 3. 89) μmol·L- 1respectively,indicating that A549 cells were 5-fold more sensitive to HCPT than MRC-5 cells at 48 h. After exposure to HCPT 50 μmol·L- 1for 48 h,some A549 cells were rounded up and shrank dramatically, and some cells underwent membrane blebbing or lysing while MRC-5 cells had no obvious changes. Cell cycle and apoptosis analysis showed that A549 cells were arrested at both S and G2/M phases and apoptosis occurred but MRC-5 cells were just arrested at S phase. In the recovery growth curve,the growth of A549 cells was inhibited to a larger extent than MRC-5 cells and the growth retardation still existed for 24 h in both cells. The survival of MRC-5 cells was faster than that of A549 cells,although there was no complete recovery in either cell. CONCLUSION A549 cells are more sensitive to HCPT than MRC-5 cells due to the fact that HCPT induces cell cycle arrest at both S and G2/M phases and apoptosis in A549 cells,but only triggers S phase arrest in the MRC-5 cells." @default.
- W2371970899 created "2016-06-24" @default.
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- W2371970899 date "2014-01-01" @default.
- W2371970899 modified "2023-09-26" @default.
- W2371970899 title "Different cytotoxic effects of hydroxycamptothecin on human lung cancer cells and human embryo lung fibroblast cells" @default.
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