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- W2384296769 abstract "Objective To construct an efficiently expressive prokaryotic clone of the second immunodominant epitopes in GP41 of HIV 1 SF2 strains. Methods According to the principle of the codon degeneracy, by PCR site directed mutagenesis, the target gene was amplified and two rare codons within it were mutated into major codons for E.coli. Then, the target DNA fragments of HIV 1 GP41 and the prokaryotic expression vector pGEX4T 2 were digested by EcoRⅠ and SalⅠ, recombined and the recombinant plasmid was then transformed into E.coli DH5α. After being induced with IPTG, the fusion protein was efficiently expressed which was identified by SDS PAGE and Western Blot. Results An efficiently expressive clone pGEX4T 2/SE/DH5α has been constructed successfully. Conclusion The second immunodominant epitopes GST fusion protein can be expressed plentifully in E.coli after being induced with IPTG." @default.
- W2384296769 created "2016-06-24" @default.
- W2384296769 creator A5081784254 @default.
- W2384296769 date "2003-01-01" @default.
- W2384296769 modified "2023-09-23" @default.
- W2384296769 title "THE SECOND IMMUNODOMINANT EPITOPES OF HIV-1 GP41: EFFICIENT EXPRESSION IN E.coli" @default.
- W2384296769 hasPublicationYear "2003" @default.
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