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- W2387253003 abstract "Objective Constructing GST-MAP3K7 fusion protein expression vector and purifying GST-MAP3K7 fusion protein to prepare for the further study of GST Pull down experiment.Methods MAP3K7 gene segments were amplified by polymerase chain reaction(PCR)and cloned into the expression vector pGEX-5X-1.Recombinant prokaryotic expression vector pGEX-5X-1-MAP3K7 was identified by restriction enzyme digestion and sequencing.Then they were transformed into E.coli BL21,induced by IPTG and purified by glutathione beads.The purified products were analyzed by SDS-PAGE and Western blot.Results The recombinant prokaryotic expression vector pGEX-5X-1-MAP3K7 had been constructed successfully.A correct specific molecular weight protein band could be seen by SDS-PAGE after GST fusion protein expression was induced.After purification,we got fusion protein with high purity.Conclusion The human MAP3K7 full length gene segments were successfully cloned into prokaryotic expression vector.The adaptive conditions for inducing the GST-MAP3K7 target protein expression were confirmed and the GST-MAP3K7 target proteins with high purity were obtained.This study provides the basis for further studies on the biological function of MAP3K7 protein." @default.
- W2387253003 created "2016-06-24" @default.
- W2387253003 creator A5012116125 @default.
- W2387253003 date "2011-01-01" @default.
- W2387253003 modified "2023-09-28" @default.
- W2387253003 title "Cloning of Human MAP3K7 Gene and Expression and Identification of GST-MAP3K7 Fusion Protein" @default.
- W2387253003 hasPublicationYear "2011" @default.
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