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- W2390513336 abstract "Objective To establish a method for simultaneous determination of methotrexate(MTX) and its 6 metabolites methotrexate polyglutamate(MTXPG) in cells by on-line sample stacking capillary electrophoresis. Methods The eletrophoresis buffer was 75 mmol/L Na2HPO4(pH=7.40).The voltage of 28 kV was applied and the injection time was 50 seconds at 0.5 psi.The separation of MTX and MTXPG was performed at a wavelength of 300 nm,and sample stacking was achieved by using distilled water and acetonitrile(V/V=1∶1) as dissolvent.Under optimal condition,intracellular MTX and MTXPG levels were detected by capillary electrophoresis.Results The optimal condition was made by adjusting isopropanol concentration(5%,8%,10%,V/V) and hp-β-CD concentration(20,25,30 mmol/L).Seven substances were completely separated by capillary electrophoresis in 30 min.A 10 fold improvement in the amount of distilled water and acetonitrile(V/V=1∶1) could be achieved by on-line sample stacking method.Conclusions MTX and MTXPG in cells can be simultaneously separated by high-performance capillary electrophoresis efficiently,rapidly,conveniently and super-sensitively." @default.
- W2390513336 created "2016-06-24" @default.
- W2390513336 creator A5058085134 @default.
- W2390513336 date "2011-01-01" @default.
- W2390513336 modified "2023-09-25" @default.
- W2390513336 title "Simultaneous determination of methotrexate and its six metabolites in cells by on-line sample stacking capillary electrophoresis" @default.
- W2390513336 hasPublicationYear "2011" @default.
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