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- W2393480306 abstract "Objective To establish a two-site immunoassay for hepatitis B e antigen(HBeAg). Methods Balb/c mice were immunized with recombinant HBeAg,and their spleen cells were fused with mouse myeloma cells SP 2/0.Cell lines secreting antibodies against two distinct antigenic determinants on HBeAg were obtained.An enzyme-linked immunosorbent assay(ELISA) kit for HBeAg was developed using a matched monoclonal antibody pair,which was compared with a commercial kit employing polyclonal and monoclonal antibody. Results 89 hybridoma cell lines secreting antibodies against HBeAg were obtained,among them 63 recognize a linear antigenic determinant and 26 recognize a conformational one.A best matched antibody pair,7E6 and e1 were selected and used to develop an ELISA kit for detecting HBeAg.Compared with the commercial kit,the monoclonal-monoclonal sandwich ELISA kit had a higher sensitivity and better specificity. Conclusions The two-site ELISA kit established for detecting HBeAg has a higher sensitivity and better specificity." @default.
- W2393480306 created "2016-06-24" @default.
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- W2393480306 date "2006-01-01" @default.
- W2393480306 modified "2023-09-23" @default.
- W2393480306 title "Establishment and application of hybridoma cell lines secreting monoclonal antibodies against two distinct antigenic determinants of hepatitis B e antigen" @default.
- W2393480306 hasPublicationYear "2006" @default.
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