Matches in SemOpenAlex for { <https://semopenalex.org/work/W2395748421> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2395748421 endingPage "28" @default.
- W2395748421 startingPage "218" @default.
- W2395748421 abstract "This study is designed to immortalize endothelial cells differentiated from embryonic stem cells. The embryoid bodies (EB) formed in vitro from embryonic stem cells, were induced to differentiate into many round cells (the precursor of endothelial cells) by retinoic acid (RA) and transforming growth factor-beta1 (TGF-beta1). These round cells later formed the vascular tube-like structures. Studies by scanning electronic microscopy and light microscopy and immunocytochemistry, demonstrated that these tube-like structures were constituted by a large number of round and flat cells, which were positive for vWF and CD34staining. These results indicate they are vascular endothelial cells. To immortalize these cells, human telomerase reverse transcriptase (hTERT) cDNA was transfected into round cells by lipofectine. hTERT mRNA expression in transfected cells was confirmed by Dot blot, RT-PCR. Furthermore, 95% these transfected cells maintain the characteristic of endothelial cell, can proliferate in large quantity in vitro, and are able to form tubular structures. These results suggest that hTERT cDNA transfection can immortalize the induced endothelial cells and therefore may provide a new source of seed cells for vascular engineering." @default.
- W2395748421 created "2016-06-24" @default.
- W2395748421 creator A5011237730 @default.
- W2395748421 creator A5015472522 @default.
- W2395748421 creator A5022944160 @default.
- W2395748421 creator A5071037763 @default.
- W2395748421 creator A5080943403 @default.
- W2395748421 creator A5082333760 @default.
- W2395748421 creator A5087748456 @default.
- W2395748421 date "2002-09-01" @default.
- W2395748421 modified "2023-10-18" @default.
- W2395748421 title "[Immortalization of endothelial cells differentiated from mouse embryonic stem cells]." @default.
- W2395748421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15344385" @default.
- W2395748421 hasPublicationYear "2002" @default.
- W2395748421 type Work @default.
- W2395748421 sameAs 2395748421 @default.
- W2395748421 citedByCount "1" @default.
- W2395748421 crossrefType "journal-article" @default.
- W2395748421 hasAuthorship W2395748421A5011237730 @default.
- W2395748421 hasAuthorship W2395748421A5015472522 @default.
- W2395748421 hasAuthorship W2395748421A5022944160 @default.
- W2395748421 hasAuthorship W2395748421A5071037763 @default.
- W2395748421 hasAuthorship W2395748421A5080943403 @default.
- W2395748421 hasAuthorship W2395748421A5082333760 @default.
- W2395748421 hasAuthorship W2395748421A5087748456 @default.
- W2395748421 hasConcept C10205521 @default.
- W2395748421 hasConcept C104317684 @default.
- W2395748421 hasConcept C123012128 @default.
- W2395748421 hasConcept C125593758 @default.
- W2395748421 hasConcept C134018914 @default.
- W2395748421 hasConcept C143948831 @default.
- W2395748421 hasConcept C145103041 @default.
- W2395748421 hasConcept C153911025 @default.
- W2395748421 hasConcept C162746124 @default.
- W2395748421 hasConcept C202751555 @default.
- W2395748421 hasConcept C20875687 @default.
- W2395748421 hasConcept C23416140 @default.
- W2395748421 hasConcept C28328180 @default.
- W2395748421 hasConcept C35866371 @default.
- W2395748421 hasConcept C54009773 @default.
- W2395748421 hasConcept C54355233 @default.
- W2395748421 hasConcept C55493867 @default.
- W2395748421 hasConcept C81885089 @default.
- W2395748421 hasConcept C86803240 @default.
- W2395748421 hasConcept C94581717 @default.
- W2395748421 hasConcept C95444343 @default.
- W2395748421 hasConceptScore W2395748421C10205521 @default.
- W2395748421 hasConceptScore W2395748421C104317684 @default.
- W2395748421 hasConceptScore W2395748421C123012128 @default.
- W2395748421 hasConceptScore W2395748421C125593758 @default.
- W2395748421 hasConceptScore W2395748421C134018914 @default.
- W2395748421 hasConceptScore W2395748421C143948831 @default.
- W2395748421 hasConceptScore W2395748421C145103041 @default.
- W2395748421 hasConceptScore W2395748421C153911025 @default.
- W2395748421 hasConceptScore W2395748421C162746124 @default.
- W2395748421 hasConceptScore W2395748421C202751555 @default.
- W2395748421 hasConceptScore W2395748421C20875687 @default.
- W2395748421 hasConceptScore W2395748421C23416140 @default.
- W2395748421 hasConceptScore W2395748421C28328180 @default.
- W2395748421 hasConceptScore W2395748421C35866371 @default.
- W2395748421 hasConceptScore W2395748421C54009773 @default.
- W2395748421 hasConceptScore W2395748421C54355233 @default.
- W2395748421 hasConceptScore W2395748421C55493867 @default.
- W2395748421 hasConceptScore W2395748421C81885089 @default.
- W2395748421 hasConceptScore W2395748421C86803240 @default.
- W2395748421 hasConceptScore W2395748421C94581717 @default.
- W2395748421 hasConceptScore W2395748421C95444343 @default.
- W2395748421 hasIssue "3" @default.
- W2395748421 hasLocation W23957484211 @default.
- W2395748421 hasOpenAccess W2395748421 @default.
- W2395748421 hasPrimaryLocation W23957484211 @default.
- W2395748421 hasRelatedWork W1490506917 @default.
- W2395748421 hasRelatedWork W2004702539 @default.
- W2395748421 hasRelatedWork W2015385107 @default.
- W2395748421 hasRelatedWork W2073521954 @default.
- W2395748421 hasRelatedWork W2079542547 @default.
- W2395748421 hasRelatedWork W2081825152 @default.
- W2395748421 hasRelatedWork W2381831260 @default.
- W2395748421 hasRelatedWork W2395748421 @default.
- W2395748421 hasRelatedWork W2411553498 @default.
- W2395748421 hasRelatedWork W2498960772 @default.
- W2395748421 hasVolume "35" @default.
- W2395748421 isParatext "false" @default.
- W2395748421 isRetracted "false" @default.
- W2395748421 magId "2395748421" @default.
- W2395748421 workType "article" @default.