Matches in SemOpenAlex for { <https://semopenalex.org/work/W2562844122> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2562844122 abstract "Abstract Hematologic side effects of cancer chemotherapy like myelosuppression are frequently dose-limiting. Lentiviral gene therapy with cytostatic drug resistance gene transfer to human hematopoietic stem cells (CD34+) is a promising approach to overcome this problem. In this context it is of interest if chemotherapy mediated selection has an impact on lentiviral integration site patterns of transduced hematopoietic stem cells (CD34+). Concerning this issue, human CD34+ cells transduced with a lentiviral self-inactivating (SIN) vector encoding MGMTP140K (the O6-BG resistant mutant of O6-methylguanine- DNA methyltransferase) were in vitro treated with the alkylating agent BCNU. For integration site analysis LM-PCR was performed and integration patterns of the treated and untreated CD34+ cells were analyzed and compared with an in silico set of 106 random integrations. We found different integration preferences of the lentiviral vector between either the treated (82 integrations) or the untreated (30 integrations) CD34+ cells and the in silico set: both groups showed chromosomal preferences, a significant bias for integrations in genes (74,4% in the treated, respectively 70% in the untreated to 40% in the in silico group), especially by favouring introns, a random integration distribution regarding transcription start sites (TSS), and most importantly no significant differences concerning the number of integrations in or near cancer genes. Concerning all integration characteristics we could not find significant differences when comparing the untreated with the treated group. In conclusion, the general distribution of lentiviral integrations in either untreated or treated human CD34+ cells showed no distinct differences between both groups but significant differences compared to the in silico integration set. These results suggest that chemoselection of cells lentivirally overexpressing a specific chemoresistence gene might not influence the integration pattern. Therefore chemotherapy pressure seems not to hamper the safety of lentiviral vectors in gene transfer studies." @default.
- W2562844122 created "2017-01-06" @default.
- W2562844122 creator A5010375570 @default.
- W2562844122 creator A5015950828 @default.
- W2562844122 creator A5025744948 @default.
- W2562844122 creator A5025849730 @default.
- W2562844122 creator A5030024392 @default.
- W2562844122 creator A5036375427 @default.
- W2562844122 creator A5042115158 @default.
- W2562844122 creator A5091140526 @default.
- W2562844122 date "2008-11-16" @default.
- W2562844122 modified "2023-09-26" @default.
- W2562844122 title "Impact of Chemoselective Pressure on Integration Site Patterns of Lentivirally Transduced Human Hematopoietic Stem Cells" @default.
- W2562844122 doi "https://doi.org/10.1182/blood.v112.11.4622.4622" @default.
- W2562844122 hasPublicationYear "2008" @default.
- W2562844122 type Work @default.
- W2562844122 sameAs 2562844122 @default.
- W2562844122 citedByCount "0" @default.
- W2562844122 crossrefType "journal-article" @default.
- W2562844122 hasAuthorship W2562844122A5010375570 @default.
- W2562844122 hasAuthorship W2562844122A5015950828 @default.
- W2562844122 hasAuthorship W2562844122A5025744948 @default.
- W2562844122 hasAuthorship W2562844122A5025849730 @default.
- W2562844122 hasAuthorship W2562844122A5030024392 @default.
- W2562844122 hasAuthorship W2562844122A5036375427 @default.
- W2562844122 hasAuthorship W2562844122A5042115158 @default.
- W2562844122 hasAuthorship W2562844122A5091140526 @default.
- W2562844122 hasConcept C10205521 @default.
- W2562844122 hasConcept C104317684 @default.
- W2562844122 hasConcept C109159458 @default.
- W2562844122 hasConcept C111599444 @default.
- W2562844122 hasConcept C153911025 @default.
- W2562844122 hasConcept C2775905019 @default.
- W2562844122 hasConcept C28328180 @default.
- W2562844122 hasConcept C32470452 @default.
- W2562844122 hasConcept C40767141 @default.
- W2562844122 hasConcept C502942594 @default.
- W2562844122 hasConcept C54355233 @default.
- W2562844122 hasConcept C86803240 @default.
- W2562844122 hasConceptScore W2562844122C10205521 @default.
- W2562844122 hasConceptScore W2562844122C104317684 @default.
- W2562844122 hasConceptScore W2562844122C109159458 @default.
- W2562844122 hasConceptScore W2562844122C111599444 @default.
- W2562844122 hasConceptScore W2562844122C153911025 @default.
- W2562844122 hasConceptScore W2562844122C2775905019 @default.
- W2562844122 hasConceptScore W2562844122C28328180 @default.
- W2562844122 hasConceptScore W2562844122C32470452 @default.
- W2562844122 hasConceptScore W2562844122C40767141 @default.
- W2562844122 hasConceptScore W2562844122C502942594 @default.
- W2562844122 hasConceptScore W2562844122C54355233 @default.
- W2562844122 hasConceptScore W2562844122C86803240 @default.
- W2562844122 hasLocation W25628441221 @default.
- W2562844122 hasOpenAccess W2562844122 @default.
- W2562844122 hasPrimaryLocation W25628441221 @default.
- W2562844122 hasRelatedWork W1973444244 @default.
- W2562844122 hasRelatedWork W1985908973 @default.
- W2562844122 hasRelatedWork W1993552958 @default.
- W2562844122 hasRelatedWork W1998704599 @default.
- W2562844122 hasRelatedWork W2014964804 @default.
- W2562844122 hasRelatedWork W2031236364 @default.
- W2562844122 hasRelatedWork W2043498273 @default.
- W2562844122 hasRelatedWork W2058523496 @default.
- W2562844122 hasRelatedWork W2058800824 @default.
- W2562844122 hasRelatedWork W2079753070 @default.
- W2562844122 hasRelatedWork W2081100565 @default.
- W2562844122 hasRelatedWork W2094959071 @default.
- W2562844122 hasRelatedWork W2120262724 @default.
- W2562844122 hasRelatedWork W2142518997 @default.
- W2562844122 hasRelatedWork W2550973346 @default.
- W2562844122 hasRelatedWork W2743269485 @default.
- W2562844122 hasRelatedWork W2765254577 @default.
- W2562844122 hasRelatedWork W2981209274 @default.
- W2562844122 hasRelatedWork W2993376952 @default.
- W2562844122 hasRelatedWork W2539389211 @default.
- W2562844122 isParatext "false" @default.
- W2562844122 isRetracted "false" @default.
- W2562844122 magId "2562844122" @default.
- W2562844122 workType "article" @default.