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• W2573183265 abstract "Essentials Recombinant factor VIII (FVIII) is known to be expressed at a low level in cell culture. To increase expression, we used codon-optimization of a B-domain deleted FVIII (BDD-FVIII). This resulted in 7-fold increase of the expression level in cell culture. The biochemical properties of codon-optimized BDD-FVIII were similar to the wild-type protein.Background Production of recombinant factor VIII (FVIII) is challenging because of its low expression. It was previously shown that codon-optimization of a B-domain-deleted FVIII (BDD-FVIII) cDNA resulted in increased protein expression. However, it is well recognized that synonymous mutations may affect the protein structure and function. Objectives To compare biochemical properties of a BDD-FVIII variants expressed from codon-optimized and wild-type cDNAs (CO and WT, respectively). Methods Each variant of the BDD-FVIII was expressed in several independent Chinese hamster ovary (CHO) cell lines, generated using a lentiviral platform. The proteins were purified by two-step affinity chromatography and analyzed in parallel by PAGE-western blot, mass spectrometry, circular dichroism, surface plasmon resonance, and chromogenic, clotting and thrombin generation assays. Results and conclusion The average yield of the CO was 7-fold higher than WT, whereas both proteins were identical in the amino acid sequences (99% coverage) and very similar in patterns of the molecular fragments (before and after thrombin cleavage), glycosylation and tyrosine sulfation, secondary structures and binding to von Willebrand factor and to a fragment of the low-density lipoprotein receptor-related protein 1. The CO preparations had on average 1.5-fold higher FVIII specific activity (activity normalized to protein mass) than WT preparations, which was attributed to better preservation of the CO structure as a result of considerably higher protein concentrations during the production. We concluded that the codon-optimization of the BDD-FVIII resulted in significant increase of its expression and did not affect the structure-function properties." @default.
• W2573183265 created "2017-01-26" @default.
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• W2573183265 date "2017-04-01" @default.
• W2573183265 modified "2023-10-16" @default.
• W2573183265 title "Expression and characterization of a codon‐optimized blood coagulation factor VIII" @default.
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• W2573183265 cites W1512481003 @default.
• W2573183265 cites W1540151595 @default.
• W2573183265 cites W1595085358 @default.
• W2573183265 cites W1896702999 @default.
• W2573183265 cites W1923501574 @default.
• W2573183265 cites W1964957928 @default.
• W2573183265 cites W1967104104 @default.
• W2573183265 cites W1967949780 @default.
• W2573183265 cites W1973402866 @default.
• W2573183265 cites W1975453558 @default.
• W2573183265 cites W1977781440 @default.
• W2573183265 cites W1982845146 @default.
• W2573183265 cites W1983094339 @default.
• W2573183265 cites W1986705405 @default.
• W2573183265 cites W1986993620 @default.
• W2573183265 cites W1991453997 @default.
• W2573183265 cites W1994167002 @default.
• W2573183265 cites W1996983268 @default.
• W2573183265 cites W1999619447 @default.
• W2573183265 cites W2002999243 @default.
• W2573183265 cites W2005058131 @default.
• W2573183265 cites W2005844368 @default.
• W2573183265 cites W2006765654 @default.
• W2573183265 cites W2007429292 @default.
• W2573183265 cites W2009916885 @default.
• W2573183265 cites W2016112642 @default.
• W2573183265 cites W2019577213 @default.
• W2573183265 cites W2020986955 @default.
• W2573183265 cites W2021283345 @default.
• W2573183265 cites W2024266527 @default.
• W2573183265 cites W2032227859 @default.
• W2573183265 cites W2034050283 @default.
• W2573183265 cites W2044693511 @default.
• W2573183265 cites W2049786448 @default.
• W2573183265 cites W2051757097 @default.
• W2573183265 cites W2054626766 @default.
• W2573183265 cites W2059951482 @default.
• W2573183265 cites W2062321149 @default.
• W2573183265 cites W2065371606 @default.
• W2573183265 cites W2066646645 @default.
• W2573183265 cites W2072287836 @default.
• W2573183265 cites W2072336384 @default.
• W2573183265 cites W2074222093 @default.
• W2573183265 cites W2079827600 @default.
• W2573183265 cites W2081398430 @default.
• W2573183265 cites W2087850432 @default.
• W2573183265 cites W2095061525 @default.
• W2573183265 cites W2096643836 @default.
• W2573183265 cites W2100421036 @default.
• W2573183265 cites W2104531050 @default.
• W2573183265 cites W2106240581 @default.
• W2573183265 cites W2118183714 @default.
• W2573183265 cites W2126189632 @default.
• W2573183265 cites W2129751025 @default.
• W2573183265 cites W2131289400 @default.
• W2573183265 cites W2146981871 @default.
• W2573183265 cites W2152001313 @default.
• W2573183265 cites W2158636606 @default.
• W2573183265 cites W2324431258 @default.
• W2573183265 cites W2334475342 @default.
• W2573183265 cites W2400075822 @default.
• W2573183265 cites W2415326267 @default.
• W2573183265 cites W2542724930 @default.
• W2573183265 cites W61301347 @default.
• W2573183265 cites W1976370439 @default.
• W2573183265 doi "https://doi.org/10.1111/jth.13632" @default.
• W2573183265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28109042" @default.
• W2573183265 hasPublicationYear "2017" @default.
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