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- W2588331863 abstract "In order to determine how heat stress affects Sertoli cells and study the type of caused damages in these cellsaffected by these conditions , this study aims at evaluating the response indicators of oxidative and the amount ofDNA damage by using the TUNEL method in Sertoli cells affected by heat stress at 42°C . Testes of 9 lambs of 3 to10months provided in slaughters and then isolation and culture of sertoli cells was performed on thesesamples.DNA damage and amount of reactive oxidative were studied in control groups under mild heat stress andextreme heat stress by using the experimental Random design and statistical test (one way ANONA ) in a statisticalsoftware SPSS. the Results of present study showed that by increasing temperature , the amount of living cellsdecreases and the difference between the percentage of living cells and cells of the control group at 32 °C with39°C groups and 42 °C groups is significant (P < 0.05 ) . Assessing by TUNEL staining showed that applying theheat stress caused an increase in the number of damaged cells from DNA and this increase was significant in 42 °Cgroup compared with control group." @default.
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- W2588331863 date "2013-01-01" @default.
- W2588331863 modified "2023-09-28" @default.
- W2588331863 title "Studying effect of heat stress on DNA damage exposure in sertoli cells" @default.
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- W2588331863 hasPublicationYear "2013" @default.
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