Matches in SemOpenAlex for { <https://semopenalex.org/work/W2766696965> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2766696965 endingPage "27" @default.
- W2766696965 startingPage "22" @default.
- W2766696965 abstract "In the present study, we report the development of a real-time PCR assay for the identification of Bacillus anthracis, based on the amplification of a unique chromosomal marker, the E4 sequence, with dual hybridization probes. The assay was evaluated using a panel of ten B. anthracis strains, two B. anthracis isolates from human clinical samples, 12 B. anthracis environmental swabs and 40 non- B. anthracis strains. All 12 B. anthracis strains and clinical isolates were correctly detected, and the method did not show cross-reactions with other micro-organisms. Likewise, the E4 sequence was not found in those strains of B. thuringiensis and B. cereus closely related (homology > 90%) to B. anthracis by computer analysis. On the other hand, this molecular assay showed a high analytical sensitivity, 3.5 genome equivalents per reaction at 95% probability. Furthermore, the real-time PCR assay allowed sequence-specific detection of the amplicon (melting peak with a Tm of 63.5 °C ± 0.5 °C) without post-amplification procedures, which offers an additional advantage over other qPCR assays for B. anthracis detection. Finally, the performance of the method was successfully evaluated in 12 environmental samples. In summary, we have developed a rapid and specific method for the molecular identification of Bacillus anthracis in environmental samples." @default.
- W2766696965 created "2017-11-10" @default.
- W2766696965 creator A5012308045 @default.
- W2766696965 creator A5019046603 @default.
- W2766696965 creator A5020937685 @default.
- W2766696965 creator A5074395917 @default.
- W2766696965 creator A5085138064 @default.
- W2766696965 date "2018-02-01" @default.
- W2766696965 modified "2023-09-26" @default.
- W2766696965 title "Rapid identification of Bacillus anthracis by real-time PCR with dual hybridization probes in environmental swabs" @default.
- W2766696965 cites W1517480469 @default.
- W2766696965 cites W1526527675 @default.
- W2766696965 cites W1602425147 @default.
- W2766696965 cites W1963563104 @default.
- W2766696965 cites W1976336995 @default.
- W2766696965 cites W1976496738 @default.
- W2766696965 cites W1985014171 @default.
- W2766696965 cites W1993257931 @default.
- W2766696965 cites W1994435497 @default.
- W2766696965 cites W2000827081 @default.
- W2766696965 cites W2003072069 @default.
- W2766696965 cites W2015241243 @default.
- W2766696965 cites W2055043387 @default.
- W2766696965 cites W2064413136 @default.
- W2766696965 cites W2070978208 @default.
- W2766696965 cites W2092939629 @default.
- W2766696965 cites W2118537438 @default.
- W2766696965 cites W2124611161 @default.
- W2766696965 cites W2135069977 @default.
- W2766696965 cites W2137734085 @default.
- W2766696965 cites W2138270253 @default.
- W2766696965 cites W2147572792 @default.
- W2766696965 cites W2161132498 @default.
- W2766696965 cites W2168420558 @default.
- W2766696965 cites W2169607526 @default.
- W2766696965 cites W2183477978 @default.
- W2766696965 cites W4254533614 @default.
- W2766696965 doi "https://doi.org/10.1016/j.mcp.2017.11.001" @default.
- W2766696965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29113932" @default.
- W2766696965 hasPublicationYear "2018" @default.
- W2766696965 type Work @default.
- W2766696965 sameAs 2766696965 @default.
- W2766696965 citedByCount "5" @default.
- W2766696965 countsByYear W27666969652019 @default.
- W2766696965 countsByYear W27666969652022 @default.
- W2766696965 countsByYear W27666969652023 @default.
- W2766696965 crossrefType "journal-article" @default.
- W2766696965 hasAuthorship W2766696965A5012308045 @default.
- W2766696965 hasAuthorship W2766696965A5019046603 @default.
- W2766696965 hasAuthorship W2766696965A5020937685 @default.
- W2766696965 hasAuthorship W2766696965A5074395917 @default.
- W2766696965 hasAuthorship W2766696965A5085138064 @default.
- W2766696965 hasConcept C104317684 @default.
- W2766696965 hasConcept C114614502 @default.
- W2766696965 hasConcept C156340839 @default.
- W2766696965 hasConcept C2777118263 @default.
- W2766696965 hasConcept C2780239966 @default.
- W2766696965 hasConcept C33923547 @default.
- W2766696965 hasConcept C49105822 @default.
- W2766696965 hasConcept C523546767 @default.
- W2766696965 hasConcept C54355233 @default.
- W2766696965 hasConcept C8185291 @default.
- W2766696965 hasConcept C86803240 @default.
- W2766696965 hasConcept C89423630 @default.
- W2766696965 hasConceptScore W2766696965C104317684 @default.
- W2766696965 hasConceptScore W2766696965C114614502 @default.
- W2766696965 hasConceptScore W2766696965C156340839 @default.
- W2766696965 hasConceptScore W2766696965C2777118263 @default.
- W2766696965 hasConceptScore W2766696965C2780239966 @default.
- W2766696965 hasConceptScore W2766696965C33923547 @default.
- W2766696965 hasConceptScore W2766696965C49105822 @default.
- W2766696965 hasConceptScore W2766696965C523546767 @default.
- W2766696965 hasConceptScore W2766696965C54355233 @default.
- W2766696965 hasConceptScore W2766696965C8185291 @default.
- W2766696965 hasConceptScore W2766696965C86803240 @default.
- W2766696965 hasConceptScore W2766696965C89423630 @default.
- W2766696965 hasLocation W27666969651 @default.
- W2766696965 hasLocation W27666969652 @default.
- W2766696965 hasOpenAccess W2766696965 @default.
- W2766696965 hasPrimaryLocation W27666969651 @default.
- W2766696965 hasRelatedWork W1971824307 @default.
- W2766696965 hasRelatedWork W2027325373 @default.
- W2766696965 hasRelatedWork W2055253926 @default.
- W2766696965 hasRelatedWork W2097355733 @default.
- W2766696965 hasRelatedWork W2108588612 @default.
- W2766696965 hasRelatedWork W2111173181 @default.
- W2766696965 hasRelatedWork W2257037517 @default.
- W2766696965 hasRelatedWork W2286322858 @default.
- W2766696965 hasRelatedWork W2766696965 @default.
- W2766696965 hasRelatedWork W3029202768 @default.
- W2766696965 hasVolume "37" @default.
- W2766696965 isParatext "false" @default.
- W2766696965 isRetracted "false" @default.
- W2766696965 magId "2766696965" @default.
- W2766696965 workType "article" @default.