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• W2940892521 abstract "Abstract An efficient PCR cloning method is indispensable in modern molecular biology, as it can greatly improve the efficiency of DNA cloning processes. Here, I describe the development of three vectors for TA cloning and blunt-end cloning. Specifically, pCRT and pCRZeroT were designed to improve the efficiency of TA cloning. pCRZeroT can also be used with pCRZero to facilitate blunt-end cloning using the ccdB gene. Using pCRZero and pCRZeroT and applying the Golden Gate reaction, I developed a direct PCR cloning protocol with non-digested circular vectors and PCR products. This direct PCR cloning protocol yielded colony-formation rates and cloning efficiencies that are comparable with those obtained by conventional PCR cloning with pre-digested vectors and PCR products. The three plasmids I designed are available from Addgene ( https://www.addgene.org/ )." @default.
• W2940892521 created "2019-05-03" @default.
• W2940892521 creator A5070445171 @default.
• W2940892521 date "2019-04-23" @default.
• W2940892521 modified "2023-09-27" @default.
• W2940892521 title "A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products" @default.
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• W2940892521 doi "https://doi.org/10.1038/s41598-019-42868-6" @default.
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