Matches in SemOpenAlex for { <https://semopenalex.org/work/W2941197405> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2941197405 endingPage "608" @default.
- W2941197405 startingPage "600" @default.
- W2941197405 abstract "Abstract Introduction Miroestrol is the potent phytoestrogen isolated from White Kwao Krua ( Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham, a Thai traditional medicinal plant. Nowadays, various health supplementary products featuring White Kwao Krua are available worldwide. A sensitive and rapid analytical method for quantification of miroestrol is necessary for quality control of these products. Objectives To prepare a single‐chain variable fragment (scFv) antibody specific to miroestrol and develop a scFv‐based enzyme‐linked immunosorbent assay (ELISA) for quantitative analysis of miroestrol in plant materials and health supplementary products. Methods A gene encoding anti‐miroestrol scFv antibody was constructed and expressed in Escherichia coli SHuffle T7 strain. Anti‐miroestrol scFv antibody was characterised and applied to ELISA. The developed scFv‐based ELISA method was validated for its sensitivity, specificity, accuracy and precision. Results Anti‐miroestrol scFv antibody was highly specific to miroestrol. The scFv‐based ELISA was applied to determine miroestrol in the range 0.06–7.81 μg/mL, with the limit of quantification of 0.06 μg/mL miroestrol. The accuracy of the assay was validated by its 95.08–103.99% recovery from the spiked miroestrol recovery experiment and in good correlation with the results from the monoclonal antibody‐based ELISA. The relative standard deviation of the intra‐ and inter‐assay were less than 6.0%. Conclusion The developed scFv‐based ELISA was sensitive, specific, accurate, and precise for determination of miroestrol and useful for quality control of P. candollei plant raw materials and supplementary products." @default.
- W2941197405 created "2019-05-03" @default.
- W2941197405 creator A5003929006 @default.
- W2941197405 creator A5014016538 @default.
- W2941197405 creator A5035356239 @default.
- W2941197405 creator A5042389027 @default.
- W2941197405 creator A5062076987 @default.
- W2941197405 creator A5067881440 @default.
- W2941197405 creator A5081096761 @default.
- W2941197405 creator A5088396413 @default.
- W2941197405 date "2019-04-25" @default.
- W2941197405 modified "2023-09-28" @default.
- W2941197405 title "Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of <i>Pueraria candollei</i> by enzyme‐linked immunosorbent assay" @default.
- W2941197405 cites W1985229180 @default.
- W2941197405 cites W1996192954 @default.
- W2941197405 cites W1996695764 @default.
- W2941197405 cites W1997437309 @default.
- W2941197405 cites W2007793265 @default.
- W2941197405 cites W2022636019 @default.
- W2941197405 cites W2024215279 @default.
- W2941197405 cites W2024668089 @default.
- W2941197405 cites W2026050785 @default.
- W2941197405 cites W2032850224 @default.
- W2941197405 cites W2049765142 @default.
- W2941197405 cites W2053479501 @default.
- W2941197405 cites W2059258455 @default.
- W2941197405 cites W2072271023 @default.
- W2941197405 cites W2073412403 @default.
- W2941197405 cites W2133117086 @default.
- W2941197405 cites W2148990116 @default.
- W2941197405 cites W2169904506 @default.
- W2941197405 cites W2177447130 @default.
- W2941197405 cites W2288340100 @default.
- W2941197405 cites W2343608303 @default.
- W2941197405 cites W2582486231 @default.
- W2941197405 cites W2587010613 @default.
- W2941197405 cites W2594746256 @default.
- W2941197405 cites W2604973989 @default.
- W2941197405 cites W2768771467 @default.
- W2941197405 cites W3023325185 @default.
- W2941197405 cites W4233090559 @default.
- W2941197405 doi "https://doi.org/10.1002/pca.2832" @default.
- W2941197405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31025473" @default.
- W2941197405 hasPublicationYear "2019" @default.
- W2941197405 type Work @default.
- W2941197405 sameAs 2941197405 @default.
- W2941197405 citedByCount "5" @default.
- W2941197405 countsByYear W29411974052020 @default.
- W2941197405 countsByYear W29411974052021 @default.
- W2941197405 countsByYear W29411974052022 @default.
- W2941197405 countsByYear W29411974052023 @default.
- W2941197405 crossrefType "journal-article" @default.
- W2941197405 hasAuthorship W2941197405A5003929006 @default.
- W2941197405 hasAuthorship W2941197405A5014016538 @default.
- W2941197405 hasAuthorship W2941197405A5035356239 @default.
- W2941197405 hasAuthorship W2941197405A5042389027 @default.
- W2941197405 hasAuthorship W2941197405A5062076987 @default.
- W2941197405 hasAuthorship W2941197405A5067881440 @default.
- W2941197405 hasAuthorship W2941197405A5081096761 @default.
- W2941197405 hasAuthorship W2941197405A5088396413 @default.
- W2941197405 hasConcept C119128265 @default.
- W2941197405 hasConcept C153911025 @default.
- W2941197405 hasConcept C159654299 @default.
- W2941197405 hasConcept C185592680 @default.
- W2941197405 hasConcept C203014093 @default.
- W2941197405 hasConcept C43617362 @default.
- W2941197405 hasConcept C542903549 @default.
- W2941197405 hasConcept C86803240 @default.
- W2941197405 hasConceptScore W2941197405C119128265 @default.
- W2941197405 hasConceptScore W2941197405C153911025 @default.
- W2941197405 hasConceptScore W2941197405C159654299 @default.
- W2941197405 hasConceptScore W2941197405C185592680 @default.
- W2941197405 hasConceptScore W2941197405C203014093 @default.
- W2941197405 hasConceptScore W2941197405C43617362 @default.
- W2941197405 hasConceptScore W2941197405C542903549 @default.
- W2941197405 hasConceptScore W2941197405C86803240 @default.
- W2941197405 hasIssue "6" @default.
- W2941197405 hasLocation W29411974051 @default.
- W2941197405 hasLocation W29411974052 @default.
- W2941197405 hasOpenAccess W2941197405 @default.
- W2941197405 hasPrimaryLocation W29411974051 @default.
- W2941197405 hasRelatedWork W1976257423 @default.
- W2941197405 hasRelatedWork W1980714464 @default.
- W2941197405 hasRelatedWork W1988317878 @default.
- W2941197405 hasRelatedWork W1999686187 @default.
- W2941197405 hasRelatedWork W2033364217 @default.
- W2941197405 hasRelatedWork W2088416340 @default.
- W2941197405 hasRelatedWork W2130473357 @default.
- W2941197405 hasRelatedWork W2368460920 @default.
- W2941197405 hasRelatedWork W2380360044 @default.
- W2941197405 hasRelatedWork W377763774 @default.
- W2941197405 hasVolume "30" @default.
- W2941197405 isParatext "false" @default.
- W2941197405 isRetracted "false" @default.
- W2941197405 magId "2941197405" @default.
- W2941197405 workType "article" @default.