FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2944566098> ?p ?o ?g. }

• W2944566098 endingPage "523" @default.
• W2944566098 startingPage "516" @default.
• W2944566098 abstract "Agrochemicals such as the non-azoles, used to improve crop productivity, poses severe undesirable effects on the environment and human health. In addition, they induce cross-resistance (CR) with clinical drugs in pathogenic fungi. However, till date emphasis has been given to the role of azoles on the induction of CR. Herein, we analyzed the effect of a non-azole agrochemical, pyraclostrobin (PCT), on the antifungal susceptibility and virulence of the human and animal pathogens Cryptococcus gattii and C. neoformans. We determined the minimum inhibitory concentration (MIC) of fluconazole (FLC), itraconazole, ravuconazole, amphotericin B, and PCT on colonies: (i) that were not exposed to PCT (non-adapted-NA-cultures), (ii) were exposed at the maximum concentration of PCT (adapted-A-cultures) and (iii) the adapted colonies after cultivation 10 times in PCT-free media (10 passages-10p–cultures). Our results showed that exposure to PCT induced both temporary and permanent CR to clinical azoles in a temperature-dependent manner. With the objective to understand the mechanism of induction of CR through non-azoles, the transcriptomes of NA and 10p cells from C. gattii R265 were analyzed. The transcriptomic analysis showed that expression of the efflux-pump genes (AFR1 and MDR1) and PCT target was higher in resistant 10p cells than that in NA. Moreover, the virulence of 10p cells was reduced as compared to NA cells in mice, as observed by the differential gene expression analysis of genes related to ion-metabolism. Additionally, we observed that FLC could not increase the survival rate of mice infected with 10p cells, confirming the occurrence of permanent CR in vivo. The findings of the present study demonstrate that the non-azole agrochemical PCT can induce permanent CR to clinical antifungals through increased expression of efflux pump genes in resistant cells and that such phenomenon also manifests in vivo." @default.
• W2944566098 created "2019-05-16" @default.
• W2944566098 creator A5003895133 @default.
• W2944566098 creator A5005880397 @default.
• W2944566098 creator A5006954173 @default.
• W2944566098 creator A5016469346 @default.
• W2944566098 creator A5043523399 @default.
• W2944566098 creator A5065799573 @default.
• W2944566098 creator A5069120270 @default.
• W2944566098 creator A5085453739 @default.
• W2944566098 creator A5087284274 @default.
• W2944566098 creator A5091611695 @default.
• W2944566098 date "2019-09-01" @default.
• W2944566098 modified "2023-10-15" @default.
• W2944566098 title "From the environment to the host: How non-azole agrochemical exposure affects the antifungal susceptibility and virulence of Cryptococcus gattii" @default.
• W2944566098 cites W1846795630 @default.
• W2944566098 cites W1964444162 @default.
• W2944566098 cites W1991731874 @default.
• W2944566098 cites W1995540241 @default.
• W2944566098 cites W2008899936 @default.
• W2944566098 cites W2014726754 @default.
• W2944566098 cites W2034241153 @default.
• W2944566098 cites W2041015559 @default.
• W2944566098 cites W2044043848 @default.
• W2944566098 cites W2055850006 @default.
• W2944566098 cites W2062709789 @default.
• W2944566098 cites W2071989458 @default.
• W2944566098 cites W2117086357 @default.
• W2944566098 cites W2126381352 @default.
• W2944566098 cites W2127366877 @default.
• W2944566098 cites W2134652427 @default.
• W2944566098 cites W2138705841 @default.
• W2944566098 cites W2144543496 @default.
• W2944566098 cites W2166862518 @default.
• W2944566098 cites W2172864837 @default.
• W2944566098 cites W2276499295 @default.
• W2944566098 cites W2337544181 @default.
• W2944566098 cites W2401831046 @default.
• W2944566098 cites W2411841804 @default.
• W2944566098 cites W2417251967 @default.
• W2944566098 cites W2508586318 @default.
• W2944566098 cites W2552182704 @default.
• W2944566098 cites W2560192804 @default.
• W2944566098 cites W2758656048 @default.
• W2944566098 cites W2767647365 @default.
• W2944566098 cites W2778173631 @default.
• W2944566098 cites W2791888954 @default.
• W2944566098 cites W2804701509 @default.
• W2944566098 cites W2890063123 @default.
• W2944566098 cites W2915739541 @default.
• W2944566098 cites W2925237293 @default.
• W2944566098 doi "https://doi.org/10.1016/j.scitotenv.2019.05.094" @default.
• W2944566098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31121401" @default.
• W2944566098 hasPublicationYear "2019" @default.
• W2944566098 type Work @default.
• W2944566098 sameAs 2944566098 @default.
• W2944566098 citedByCount "16" @default.
• W2944566098 countsByYear W29445660982020 @default.
• W2944566098 countsByYear W29445660982021 @default.
• W2944566098 countsByYear W29445660982022 @default.
• W2944566098 countsByYear W29445660982023 @default.
• W2944566098 crossrefType "journal-article" @default.
• W2944566098 hasAuthorship W2944566098A5003895133 @default.
• W2944566098 hasAuthorship W2944566098A5005880397 @default.
• W2944566098 hasAuthorship W2944566098A5006954173 @default.
• W2944566098 hasAuthorship W2944566098A5016469346 @default.
• W2944566098 hasAuthorship W2944566098A5043523399 @default.
• W2944566098 hasAuthorship W2944566098A5065799573 @default.
• W2944566098 hasAuthorship W2944566098A5069120270 @default.
• W2944566098 hasAuthorship W2944566098A5085453739 @default.
• W2944566098 hasAuthorship W2944566098A5087284274 @default.
• W2944566098 hasAuthorship W2944566098A5091611695 @default.
• W2944566098 hasBestOaLocation W29445660982 @default.
• W2944566098 hasConcept C104317684 @default.
• W2944566098 hasConcept C200082930 @default.
• W2944566098 hasConcept C2777071850 @default.
• W2944566098 hasConcept C2777893358 @default.
• W2944566098 hasConcept C2779286289 @default.
• W2944566098 hasConcept C2779548794 @default.
• W2944566098 hasConcept C2779629538 @default.
• W2944566098 hasConcept C2780315139 @default.
• W2944566098 hasConcept C2780651595 @default.
• W2944566098 hasConcept C55493867 @default.
• W2944566098 hasConcept C60987743 @default.
• W2944566098 hasConcept C86803240 @default.
• W2944566098 hasConcept C89423630 @default.
• W2944566098 hasConceptScore W2944566098C104317684 @default.
• W2944566098 hasConceptScore W2944566098C200082930 @default.
• W2944566098 hasConceptScore W2944566098C2777071850 @default.
• W2944566098 hasConceptScore W2944566098C2777893358 @default.
• W2944566098 hasConceptScore W2944566098C2779286289 @default.
• W2944566098 hasConceptScore W2944566098C2779548794 @default.
• W2944566098 hasConceptScore W2944566098C2779629538 @default.
• W2944566098 hasConceptScore W2944566098C2780315139 @default.
• W2944566098 hasConceptScore W2944566098C2780651595 @default.
• W2944566098 hasConceptScore W2944566098C55493867 @default.
• W2944566098 hasConceptScore W2944566098C60987743 @default.
• W2944566098 hasConceptScore W2944566098C86803240 @default.

• next