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• W2944904403 abstract "In this work, modular engineering of Escherichia coli was peformed to improve flavin production and the conversion ratio of riboflavin (RF) to FMN/FAD. The RF operon and the bifunctional RF kinase/FAD synthetase were divided into two separate modules. The two modules were expressed at different levels to produce RF:ribF ratios ranging from 2:20 to 7:5. The best strain respectively produced 324.1 and 171.6 mg/L of FAD and FMN in shake flask fermentation, and the titers reached 1899.3 and 872.7 mg/L in a fed-batch process. Furthermore, error-prone PCR (epPCR) of the E. coli ribF gene was performed. The highest FMN production of the best mutant reached 586.1 mg/L in shake flask cultivation. Moreover, this mutant produced 1017.5 mg/L FMN with a greatly reduced proportion of FAD in fermenter culture. To the best of our knowledge, this is the highest production of FAD and FMN in a microbial fermentation process reported to date." @default.
• W2944904403 created "2019-05-29" @default.
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• W2944904403 date "2019-05-17" @default.
• W2944904403 modified "2023-10-16" @default.
• W2944904403 title "Modular Engineering of the Flavin Pathway in <i>Escherichia coli</i> for Improved Flavin Mononucleotide and Flavin Adenine Dinucleotide Production" @default.
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• W2944904403 doi "https://doi.org/10.1021/acs.jafc.9b02646" @default.
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