Matches in SemOpenAlex for { <https://semopenalex.org/work/W2947165498> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2947165498 abstract "Single-molecule immunoassays have recently attracted wide attention due to the ability to identify and detect individual analyte molecules, which provides the ultimate sensitivity in bioanalytical applications. Popular labels for single-molecule assays are enzymes in confined volumes, however, the signal generation is time consuming, enzymes have limited stability and the supporting chips are often costly. In recent years, many techniques based on nanomaterials as detection labels have emerged, that can be used to overcome the disadvantages of enzymes and improve the assay properties. Photon-upconversion nanoparticles (UCNPs) are excellent labels for single-molecule immunoassays because they emit shorter-wavelength light under near-infrared excitation (anti-Stokes emission), which limits autofluorescence and light scattering. These unique photoluminescent features enable the detection of UCNPs at the single nanoparticle level by conventional wide-field epiluminescence microscopy. We have synthesized streptavidin-conjugated polyethylene glycol-coated UCNPs and applied them as labels for the detection of the cancer biomarker prostate specific antigen (PSA). The individual sandwich immunocomplexes were counted using a wide-field microscope equipped with a 980 nm laser excitation source. The noise-surpassing digital readout (particle counting) resulted in 20× higher sensitivity than the analog readout (emitted light intensity) and allowed to reach a detection limit of 800 aM (23 fg mL-1) in 25% serum. The streptavidin-conjugated UCNPs are also suitable as labels for detection of pathogenic bacteria. We have developed an assay based on in-house prepared antibodies for the detection of Melissococcus plutonius, causative agent of European foulbrood. The assay provided detection limit of 10^4 CFU mL-1 and its practical relevance was demonstrated by the analysis of real samples of honeybees and larvae." @default.
- W2947165498 created "2019-06-07" @default.
- W2947165498 creator A5006276698 @default.
- W2947165498 creator A5009961097 @default.
- W2947165498 creator A5011789826 @default.
- W2947165498 creator A5012600252 @default.
- W2947165498 creator A5029429628 @default.
- W2947165498 creator A5039015701 @default.
- W2947165498 creator A5079363307 @default.
- W2947165498 creator A5082301783 @default.
- W2947165498 date "2019-01-01" @default.
- W2947165498 modified "2023-09-24" @default.
- W2947165498 title "Single-molecule immunoassays based on upconversion nanoparticles for detection of cancer markers and bacteria" @default.
- W2947165498 hasPublicationYear "2019" @default.
- W2947165498 type Work @default.
- W2947165498 sameAs 2947165498 @default.
- W2947165498 citedByCount "0" @default.
- W2947165498 crossrefType "journal-article" @default.
- W2947165498 hasAuthorship W2947165498A5006276698 @default.
- W2947165498 hasAuthorship W2947165498A5009961097 @default.
- W2947165498 hasAuthorship W2947165498A5011789826 @default.
- W2947165498 hasAuthorship W2947165498A5012600252 @default.
- W2947165498 hasAuthorship W2947165498A5029429628 @default.
- W2947165498 hasAuthorship W2947165498A5039015701 @default.
- W2947165498 hasAuthorship W2947165498A5079363307 @default.
- W2947165498 hasAuthorship W2947165498A5082301783 @default.
- W2947165498 hasConcept C10390740 @default.
- W2947165498 hasConcept C11630735 @default.
- W2947165498 hasConcept C119128265 @default.
- W2947165498 hasConcept C120665830 @default.
- W2947165498 hasConcept C121332964 @default.
- W2947165498 hasConcept C147080431 @default.
- W2947165498 hasConcept C148869448 @default.
- W2947165498 hasConcept C155672457 @default.
- W2947165498 hasConcept C160756335 @default.
- W2947165498 hasConcept C171250308 @default.
- W2947165498 hasConcept C179687394 @default.
- W2947165498 hasConcept C185592680 @default.
- W2947165498 hasConcept C192562407 @default.
- W2947165498 hasConcept C2776190903 @default.
- W2947165498 hasConcept C2778204606 @default.
- W2947165498 hasConcept C31332276 @default.
- W2947165498 hasConcept C43617362 @default.
- W2947165498 hasConcept C49040817 @default.
- W2947165498 hasConcept C55493867 @default.
- W2947165498 hasConcept C67649825 @default.
- W2947165498 hasConcept C91881484 @default.
- W2947165498 hasConceptScore W2947165498C10390740 @default.
- W2947165498 hasConceptScore W2947165498C11630735 @default.
- W2947165498 hasConceptScore W2947165498C119128265 @default.
- W2947165498 hasConceptScore W2947165498C120665830 @default.
- W2947165498 hasConceptScore W2947165498C121332964 @default.
- W2947165498 hasConceptScore W2947165498C147080431 @default.
- W2947165498 hasConceptScore W2947165498C148869448 @default.
- W2947165498 hasConceptScore W2947165498C155672457 @default.
- W2947165498 hasConceptScore W2947165498C160756335 @default.
- W2947165498 hasConceptScore W2947165498C171250308 @default.
- W2947165498 hasConceptScore W2947165498C179687394 @default.
- W2947165498 hasConceptScore W2947165498C185592680 @default.
- W2947165498 hasConceptScore W2947165498C192562407 @default.
- W2947165498 hasConceptScore W2947165498C2776190903 @default.
- W2947165498 hasConceptScore W2947165498C2778204606 @default.
- W2947165498 hasConceptScore W2947165498C31332276 @default.
- W2947165498 hasConceptScore W2947165498C43617362 @default.
- W2947165498 hasConceptScore W2947165498C49040817 @default.
- W2947165498 hasConceptScore W2947165498C55493867 @default.
- W2947165498 hasConceptScore W2947165498C67649825 @default.
- W2947165498 hasConceptScore W2947165498C91881484 @default.
- W2947165498 hasLocation W29471654981 @default.
- W2947165498 hasOpenAccess W2947165498 @default.
- W2947165498 hasPrimaryLocation W29471654981 @default.
- W2947165498 hasRelatedWork W2018164898 @default.
- W2947165498 hasRelatedWork W2079965490 @default.
- W2947165498 hasRelatedWork W2091886017 @default.
- W2947165498 hasRelatedWork W2160725129 @default.
- W2947165498 hasRelatedWork W2304608681 @default.
- W2947165498 hasRelatedWork W2405057777 @default.
- W2947165498 hasRelatedWork W2757348710 @default.
- W2947165498 hasRelatedWork W2792323088 @default.
- W2947165498 hasRelatedWork W2911851389 @default.
- W2947165498 hasRelatedWork W2915924605 @default.
- W2947165498 hasRelatedWork W2937382900 @default.
- W2947165498 hasRelatedWork W2952163070 @default.
- W2947165498 hasRelatedWork W2955144726 @default.
- W2947165498 hasRelatedWork W3013695655 @default.
- W2947165498 hasRelatedWork W3066032836 @default.
- W2947165498 hasRelatedWork W3107120280 @default.
- W2947165498 hasRelatedWork W3108365093 @default.
- W2947165498 hasRelatedWork W3179777039 @default.
- W2947165498 hasRelatedWork W3187865705 @default.
- W2947165498 hasRelatedWork W3210415573 @default.
- W2947165498 isParatext "false" @default.
- W2947165498 isRetracted "false" @default.
- W2947165498 magId "2947165498" @default.
- W2947165498 workType "article" @default.