FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2949673053> ?p ?o ?g. }

• W2949673053 endingPage "2875" @default.
• W2949673053 startingPage "2862" @default.
• W2949673053 abstract "Abstract Y257 of Oryza sativa parkeol synthase ( Os OSC2) corresponds to H234 of Saccharomyces cerevisiae lanosterol cyclase ( Sc LAS), which is believed to be responsible for the final deprotonation reaction. An Ala mutant afforded nine tetracyclic skeletons as the main products; they consisted of protostadie n ol scaffolds with both 17 R and 17 S configurations and both 20 R and 20 S configurations, as well as a pair of 20 R ‐ and 20 S ‐configured parkeols. The production of 20 R ‐ and 20 S ‐configured tetracycles (59:40 ratio) through the action of the Y257A mutant indicated that the substrate folding had been altered from a chair–boat–chair– chair (a normal folding pattern) to a chair–boat–chair– boat structure (an unusual folding pattern). Other mutants with different steric bulks also yielded both 20 R ‐ and 20 S ‐configured tetracycles. Thus, the primary function of Y257 appears to be to impose a normal chair structure at the D‐ring site through having appropriate steric bulk. In contrast, mutations at H234 of Sc LAS were reported to cause no conformational changes. The Os OSC2 Phe mutant also yielded 20 R ‐ and 20 S ‐configured parkeols (25:33 ratio), thus suggesting that the OH group of Y257 can form hydrogen bonds with other amino acids to force a chair conformation at the D‐ring site, and this variant also gave 20 R ‐ and 20 S ‐configured parkeols in a high yield (60 %). Y257 is unlikely to act as a base to abstract H‐11 and stabilize the transient cation through cation–π interactions. Thus, the catalytic roles of Y257 are significantly different from those of H234 of Sc LAS." @default.
• W2949673053 created "2019-06-27" @default.
• W2949673053 creator A5042274769 @default.
• W2949673053 creator A5050813553 @default.
• W2949673053 creator A5056901462 @default.
• W2949673053 creator A5083318297 @default.
• W2949673053 date "2019-10-15" @default.
• W2949673053 modified "2023-09-28" @default.
• W2949673053 title "<i>Oryza sativa</i> Parkeol Cyclase: Changes in the Substrate‐Folding Conformation and the Deprotonation Sites on Mutation at Tyr257: Importance of the Hydroxy Group and Steric Bulk" @default.
• W2949673053 cites W1929126126 @default.
• W2949673053 cites W1987126722 @default.
• W2949673053 cites W1988256563 @default.
• W2949673053 cites W1995681725 @default.
• W2949673053 cites W2002470296 @default.
• W2949673053 cites W2009037782 @default.
• W2949673053 cites W2009620413 @default.
• W2949673053 cites W2028331999 @default.
• W2949673053 cites W2031264029 @default.
• W2949673053 cites W2034017325 @default.
• W2949673053 cites W2037736962 @default.
• W2949673053 cites W2041790732 @default.
• W2949673053 cites W2048382801 @default.
• W2949673053 cites W2053860436 @default.
• W2949673053 cites W2058233843 @default.
• W2949673053 cites W2069854611 @default.
• W2949673053 cites W2070456262 @default.
• W2949673053 cites W2070755711 @default.
• W2949673053 cites W2075602575 @default.
• W2949673053 cites W2083895917 @default.
• W2949673053 cites W2086268087 @default.
• W2949673053 cites W2086548308 @default.
• W2949673053 cites W2086757808 @default.
• W2949673053 cites W2091791271 @default.
• W2949673053 cites W2102968079 @default.
• W2949673053 cites W2106855080 @default.
• W2949673053 cites W2125054737 @default.
• W2949673053 cites W2131855365 @default.
• W2949673053 cites W2165218676 @default.
• W2949673053 cites W2166813071 @default.
• W2949673053 cites W2332643405 @default.
• W2949673053 cites W2428396425 @default.
• W2949673053 cites W2461919869 @default.
• W2949673053 cites W2592909587 @default.
• W2949673053 cites W2789478322 @default.
• W2949673053 cites W2807604829 @default.
• W2949673053 cites W2949837111 @default.
• W2949673053 cites W2953280730 @default.
• W2949673053 cites W4210570158 @default.
• W2949673053 doi "https://doi.org/10.1002/cbic.201900314" @default.
• W2949673053 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31180162" @default.
• W2949673053 hasPublicationYear "2019" @default.
• W2949673053 type Work @default.
• W2949673053 sameAs 2949673053 @default.
• W2949673053 citedByCount "3" @default.
• W2949673053 countsByYear W29496730532021 @default.
• W2949673053 countsByYear W29496730532023 @default.
• W2949673053 crossrefType "journal-article" @default.
• W2949673053 hasAuthorship W2949673053A5042274769 @default.
• W2949673053 hasAuthorship W2949673053A5050813553 @default.
• W2949673053 hasAuthorship W2949673053A5056901462 @default.
• W2949673053 hasAuthorship W2949673053A5083318297 @default.
• W2949673053 hasConcept C104317684 @default.
• W2949673053 hasConcept C118629725 @default.
• W2949673053 hasConcept C119599485 @default.
• W2949673053 hasConcept C127413603 @default.
• W2949673053 hasConcept C143065580 @default.
• W2949673053 hasConcept C145148216 @default.
• W2949673053 hasConcept C178790620 @default.
• W2949673053 hasConcept C185592680 @default.
• W2949673053 hasConcept C201194858 @default.
• W2949673053 hasConcept C2776545253 @default.
• W2949673053 hasConcept C2780378348 @default.
• W2949673053 hasConcept C55493867 @default.
• W2949673053 hasConcept C71240020 @default.
• W2949673053 hasConcept C8010536 @default.
• W2949673053 hasConceptScore W2949673053C104317684 @default.
• W2949673053 hasConceptScore W2949673053C118629725 @default.
• W2949673053 hasConceptScore W2949673053C119599485 @default.
• W2949673053 hasConceptScore W2949673053C127413603 @default.
• W2949673053 hasConceptScore W2949673053C143065580 @default.
• W2949673053 hasConceptScore W2949673053C145148216 @default.
• W2949673053 hasConceptScore W2949673053C178790620 @default.
• W2949673053 hasConceptScore W2949673053C185592680 @default.
• W2949673053 hasConceptScore W2949673053C201194858 @default.
• W2949673053 hasConceptScore W2949673053C2776545253 @default.
• W2949673053 hasConceptScore W2949673053C2780378348 @default.
• W2949673053 hasConceptScore W2949673053C55493867 @default.
• W2949673053 hasConceptScore W2949673053C71240020 @default.
• W2949673053 hasConceptScore W2949673053C8010536 @default.
• W2949673053 hasIssue "22" @default.
• W2949673053 hasLocation W29496730531 @default.
• W2949673053 hasLocation W29496730532 @default.
• W2949673053 hasOpenAccess W2949673053 @default.
• W2949673053 hasPrimaryLocation W29496730531 @default.
• W2949673053 hasRelatedWork W2012602682 @default.
• W2949673053 hasRelatedWork W2065862411 @default.
• W2949673053 hasRelatedWork W2102293398 @default.
• W2949673053 hasRelatedWork W2344209642 @default.

• next