Matches in SemOpenAlex for { <https://semopenalex.org/work/W2968683279> ?p ?o ?g. }
- W2968683279 endingPage "212" @default.
- W2968683279 startingPage "201" @default.
- W2968683279 abstract "The use of mixed pastures of grasses with forage peanut (Arachis pintoi) is growing and offers promising results. This leguminous forage plant has a stoloniferous growth habit and tolerates more intense defoliation and shading. However, there are few studies on its symbiosis with nitrogen (N2) fixing bacteria and no studies on genetic identification of its native microsymbionts. The aim of this study was to evaluate phenotypic and genetic diversity and the symbiotic efficiency of bacterial strains isolated from nodules of forage peanut intercropped with grasses in pasture areas. Root nodules were collected from the Belomonte cultivar in Itabela, Bahia, and BRS Mandobi cultivar in Lavras, Minas Gerais, Brazil. The areas of sample collection had contrasting physical and chemical characteristics. Samples of 10 plants per area and 5 nodules per plant were taken. The strains isolated from the nodules were analyzed for culture characteristics (growth period and change in pH of the culture medium), their 16S rRNA, gyrB and nifH genes were partially sequenced, and authentication and analysis of symbiotic efficiency in forage peanut plants were carried out. Both the authentication and symbiotic efficiency experiments were carried out in a greenhouse under axenic conditions, using A. pintoi cv. BRS Mandobi in a completely randomized design with four replications. Authentication was performed in long neck bottles (500 mL) filled with nutrient solution, and symbiotic efficiency was performed in polypropylene seedling plug pots containing a mixture of sand and vermiculite with a nutrient solution. A total of 74 bacterial strains were obtained from isolation of the forage peanut nodules, 40 from Itabela, and 34 from Lavras. Partial sequencing of the 16S rRNA gene was obtained for 48 strains, 30 from Itabela, and 18 from Lavras. The strains isolated from nodules of forage peanut in pasture areas of Itabela, were identified in the following genera: Bradyrhizobium (21), Rhizobium (5), Burkholderia (2), Kocuria (1), and Paenibacillus (1). The strains coming from soils of Lavras, were identified in the following genera: Bradyrhizobium (13), Rhizobium (2), Burkholderia (1), Mucilaginibacter (1), and Enterobacter (1). In the authentication experiment, 17 strains from Itabela, and 13 strains from Lavras, exhibited nodulation. From these authenticated strains, 18 were identified by 16S rRNA sequencing as belonging to the Bradyrhizobium genus. Of these strains, 11 from the two areas exhibited high symbiotic efficiency in A. pintoi, and their results were better than those of the strains currently approved as inoculants for this crop. The non-nodulating strains are probably nodule endophytes. Sequencing of the gyrB housekeeping gene, performed in 13 strains, confirms phylogenetic groups of potential new species among the strains isolated from A. pintoi, which include strains able to nodulate this species, belonging to a specific phylogenetic group of Bradyrhizobium. Sequences of nifH gene of efficient strains belonged to a group different from that formed by non-nodulating strains." @default.
- W2968683279 created "2019-08-22" @default.
- W2968683279 creator A5013442543 @default.
- W2968683279 creator A5014533316 @default.
- W2968683279 creator A5022744015 @default.
- W2968683279 creator A5043996160 @default.
- W2968683279 creator A5056226921 @default.
- W2968683279 creator A5075079825 @default.
- W2968683279 date "2019-11-01" @default.
- W2968683279 modified "2023-09-26" @default.
- W2968683279 title "Microsymbionts of forage peanut under different soil and climate conditions belong to a specific group of Bradyrhizobium strains" @default.
- W2968683279 cites W1603863371 @default.
- W2968683279 cites W1747388711 @default.
- W2968683279 cites W1874227847 @default.
- W2968683279 cites W1972843561 @default.
- W2968683279 cites W1974643812 @default.
- W2968683279 cites W1979690942 @default.
- W2968683279 cites W1988517056 @default.
- W2968683279 cites W1991368592 @default.
- W2968683279 cites W2001204302 @default.
- W2968683279 cites W2005475199 @default.
- W2968683279 cites W2016059598 @default.
- W2968683279 cites W2016377827 @default.
- W2968683279 cites W2016451488 @default.
- W2968683279 cites W2028781065 @default.
- W2968683279 cites W2044134971 @default.
- W2968683279 cites W2065461553 @default.
- W2968683279 cites W2075703843 @default.
- W2968683279 cites W2084044509 @default.
- W2968683279 cites W2089572093 @default.
- W2968683279 cites W2090717088 @default.
- W2968683279 cites W2093054805 @default.
- W2968683279 cites W2107477074 @default.
- W2968683279 cites W2121249856 @default.
- W2968683279 cites W2131333213 @default.
- W2968683279 cites W2132632499 @default.
- W2968683279 cites W2132666490 @default.
- W2968683279 cites W2137220016 @default.
- W2968683279 cites W2149410361 @default.
- W2968683279 cites W2149544070 @default.
- W2968683279 cites W2154221975 @default.
- W2968683279 cites W2159446274 @default.
- W2968683279 cites W2164769751 @default.
- W2968683279 cites W2170347625 @default.
- W2968683279 cites W2761581123 @default.
- W2968683279 cites W2764293474 @default.
- W2968683279 cites W2808257986 @default.
- W2968683279 cites W2937024333 @default.
- W2968683279 cites W885045794 @default.
- W2968683279 cites W925646174 @default.
- W2968683279 doi "https://doi.org/10.1016/j.apsoil.2019.07.018" @default.
- W2968683279 hasPublicationYear "2019" @default.
- W2968683279 type Work @default.
- W2968683279 sameAs 2968683279 @default.
- W2968683279 citedByCount "8" @default.
- W2968683279 countsByYear W29686832792020 @default.
- W2968683279 countsByYear W29686832792021 @default.
- W2968683279 countsByYear W29686832792022 @default.
- W2968683279 crossrefType "journal-article" @default.
- W2968683279 hasAuthorship W2968683279A5013442543 @default.
- W2968683279 hasAuthorship W2968683279A5014533316 @default.
- W2968683279 hasAuthorship W2968683279A5022744015 @default.
- W2968683279 hasAuthorship W2968683279A5043996160 @default.
- W2968683279 hasAuthorship W2968683279A5056226921 @default.
- W2968683279 hasAuthorship W2968683279A5075079825 @default.
- W2968683279 hasConcept C126574351 @default.
- W2968683279 hasConcept C144027150 @default.
- W2968683279 hasConcept C180032290 @default.
- W2968683279 hasConcept C181440489 @default.
- W2968683279 hasConcept C197321923 @default.
- W2968683279 hasConcept C2777465193 @default.
- W2968683279 hasConcept C2779370140 @default.
- W2968683279 hasConcept C2780172293 @default.
- W2968683279 hasConcept C2780434414 @default.
- W2968683279 hasConcept C523546767 @default.
- W2968683279 hasConcept C54355233 @default.
- W2968683279 hasConcept C6557445 @default.
- W2968683279 hasConcept C86803240 @default.
- W2968683279 hasConceptScore W2968683279C126574351 @default.
- W2968683279 hasConceptScore W2968683279C144027150 @default.
- W2968683279 hasConceptScore W2968683279C180032290 @default.
- W2968683279 hasConceptScore W2968683279C181440489 @default.
- W2968683279 hasConceptScore W2968683279C197321923 @default.
- W2968683279 hasConceptScore W2968683279C2777465193 @default.
- W2968683279 hasConceptScore W2968683279C2779370140 @default.
- W2968683279 hasConceptScore W2968683279C2780172293 @default.
- W2968683279 hasConceptScore W2968683279C2780434414 @default.
- W2968683279 hasConceptScore W2968683279C523546767 @default.
- W2968683279 hasConceptScore W2968683279C54355233 @default.
- W2968683279 hasConceptScore W2968683279C6557445 @default.
- W2968683279 hasConceptScore W2968683279C86803240 @default.
- W2968683279 hasFunder F4320321091 @default.
- W2968683279 hasFunder F4320322025 @default.
- W2968683279 hasFunder F4320322980 @default.
- W2968683279 hasLocation W29686832791 @default.
- W2968683279 hasOpenAccess W2968683279 @default.
- W2968683279 hasPrimaryLocation W29686832791 @default.
- W2968683279 hasRelatedWork W1601790068 @default.