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• W2979734423 startingPage "120436" @default.
• W2979734423 abstract "pH regulates the function of many organelles and plays a pivotal role in requiring multitud cellular behaviors. Compared with single fluorescent probes, ratio fluorescent probes have higher sensitivity and immunity to interference. Herein, a novel Janus ratio nanoprobe was developed for intracellular pH detection. Modified rhodamine B probe and fluorescein isothiocyanate (FITC) were individually encapsulated in the independent hemispheres of Janus microparticles fabricated via Pickering emulsion. Moreover, it exhibits a satasified ratiometric detection of pH compared to the previous core-shell structure and organic small molecule probe. Accordingly, the Janus nanoprobe possesses many important features as an attractive sensor, including high anti-jamming capability, excellent stability, good reversibility and low cytotoxicity. Variations of the two fluorescence intensities (Fgreen/Fred) resulted in a ratiometric pH fluorescent sensor, which can respond to wide range of pH values from 3 to 8. To be more specific, with a single excitation wavelength of 488 nm, there are dual emission bands centered at 538 nm and 590 nm. Also the Janus nanoprobe displays a excellent linear relationship in the physiologically relevant pH range of 4.0–6.0. Consequently, detecting of pH and imaging was successfully achieved in living cells, which provides a simple and reliable method for detecting intracelluar pH and other similar substances." @default.
• W2979734423 created "2019-10-18" @default.
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• W2979734423 date "2020-03-01" @default.
• W2979734423 modified "2023-10-18" @default.
• W2979734423 title "A novel cell-penetrating Janus nanoprobe for ratiometric fluorescence detection of pH in living cells" @default.
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• W2979734423 doi "https://doi.org/10.1016/j.talanta.2019.120436" @default.
• W2979734423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31892062" @default.
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