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- W3004528162 abstract "Microvesicles released from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sa-MVs) contain a tough membrane made exclusively of tetraether lipids and are covered by S-layer proteins. Sa-MVs have been implicated in many archaea physiological functions and are potentially useful for biotech applications. However, little has been done to exploit the physical properties of Sa-MVs. We used fluorescence spectroscopy, light scattering, laser Doppler electrophoresis. and cryo-transmission electron microscopy to study the stability, morphology, dynamic structure, and packing properties of Sa-MVs. Our findings are as follows: Sa-MVs have long term (> 4.5 months) stability when stored at room temperature; Under physiological growth conditions (75oC, pH 2.6), the average size of Sa-MVs is ∼180 nm, which remains unchanged in the temperature range examined (25-80oC); When the pH increases from 2.6 to 7.2, Sa-MVs decrease their size by ∼40 nm, via protein dependent events; The isoelectric point for Sa-MVs in 1 mM KCl is 3.0 while that for liposomes reconstituted from Sa-MV lipids (LUVMV) is below 2.0. Sa-MVs are stable against autoclaving at neutral pH in terms of size and shape; The emission maximum of Sa-MV intrinsic protein fluorescence appears at 296-303 nm, which is one of the bluest ever reported, indicative of very tight packing in the protein matrix; when S-layer proteins are aggregated as a crystalline structure on the surface of Sa-MVs, the excitation spectrum of Sa-MV intrinsic protein fluorescence undergoes a red shift, along with an increase in excitation band sharpness, indicating J-aggregate formation; and the red edge excitation shift (REES) of Laurdan fluorescence in Sa-MVs is much higher than that seen in diester liposomes, indicating that Laurdan in Sa-MVs is in a very motionally restricted environment. In conclusion, Sa-MVs are naturally occurring nanoparticles extraordinarily stable against environmental stressors and unusually tightly packed. (Supported by NSF)." @default.
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- W3004528162 date "2020-02-01" @default.
- W3004528162 modified "2023-09-27" @default.
- W3004528162 title "Sulfolobus acidocaldarius Microvesicles Exhibit Unusual Physical Properties" @default.
- W3004528162 doi "https://doi.org/10.1016/j.bpj.2019.11.591" @default.
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