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• W3034503582 endingPage "30889" @default.
• W3034503582 startingPage "30882" @default.
• W3034503582 abstract "In this work, we proposed a new strategy of fabricating time-resolved fluorescent nanoprobes by using an enzyme-integrated lanthanide coordination polymer (CP) composite for the detection of superoxide anions (O2•–). This CP composite was constructed with terbium ions (Tb3+) as a metal node, adenosine triphosphate (ATP) as a bridge ligand, and carboxyphenylboronic acid (CPBA) as a sensitizer in which superoxide dismutase (SOD) was encapsulated by a self-adaptive inclusion process. The as-prepared SOD@ATP/Tb-CPBA displays both catalytic and fluorescence properties. Benefiting from the shielding effect of ATP/Tb CP, greatly enhanced catalytic activity and stability against harsh environments can be obtained in the loaded SOD. Meanwhile, the loaded SOD can remove the water molecules on the coordination sphere of Tb3+, leading to a significant increase in the fluorescence intensity and lifetime of SOD@ATP/Tb-CPBA. However, upon the addition of O2•–, the fluorescence of SOD@ATP/Tb-CPBA was quenched significantly. This is because SOD can convert O2•– into H2O2 to induce the deboronation of CPBA, resulting in an intramolecular charge transfer process. On this basis, by taking advantage of Tb3+ in long lifetime emission, a time-resolved fluorescence method was developed for the detection of O2•–, and satisfactory results have been achieved in both buffered aqueous solutions and serum samples. We believe that the presented study will open up a new avenue to develop enzyme-involved fluorescent nanoprobes." @default.
• W3034503582 created "2020-06-19" @default.
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• W3034503582 date "2020-06-11" @default.
• W3034503582 modified "2023-09-27" @default.
• W3034503582 title "Time-Resolved Fluorescence Detection of Superoxide Anions Based on an Enzyme-Integrated Lanthanide Coordination Polymer Composite" @default.
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• W3034503582 doi "https://doi.org/10.1021/acsami.0c09080" @default.
• W3034503582 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32525648" @default.
• W3034503582 hasPublicationYear "2020" @default.
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