FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3039750872> ?p ?o ?g. }

• W3039750872 endingPage "4249" @default.
• W3039750872 startingPage "4239" @default.
• W3039750872 abstract "The development of precision medicine needs a novel genetic screening and diagnostic technique for clinical detection. This study aims to establish a method for highly parallel multiplexed detection of genetic mutations in Chinese lung cancer samples through testing 285 genes by customized next generation sequencing (NGS) on Ion-Proton platform.We reviewed the related literature and collected data of genomic alteration that occurred in lung cancer. We identified 285 target genes closely related to the pathogenesis, drug resistance, and metastasis of lung cancer. Targeted hybridization probes were designed using SureDesign software. The detection method was established by analyzing four cell lines and 13 lung cancer specimens which had been validated through Sanger sequencing. The sensitivity and specificity of the proposed method were preliminarily evaluated by comparisons with the Sanger sequencing and a LungCarta mutation-detection method.The proposed method was able to detect mutations of 285 genes in lung cancer cell lines and clinical lung cancer specimens. The reads, mapped reads, on target, mean depth and uniformity were 14.90±4.37 (×106), 98.68%±0.61%, 60.49%±10.72%, 714.42±264.13 and 90.51%±6.91%, respectively. The detected mutation result of cell lines was consistent with the observations on previously reported mutations, and the congruence rate was 100%. The proposed method can detect single nucleotide polymorphism (SNP), InDel, Fusion and copy number variation (CNV). The complete congruence rate of detected result of specimens between the proposed method and Sanger sequencing, LungCarta mutation-detection method, immunohistochemistry (IHC), real-time polymerase chain reaction (RT-PCR) method were all 100% regarding mutations in common genes like EGFR, KRAS, or fusions of ALK, RET, etc. In addition, NFE2L3_p.Ser511_Pro513del, ERBB2_E770delinsEAYVM, MET_S701N, PDGFRA_T674I, TP53_G245V, TP53_V274A, TP53_A276F, TP53_G334L, TP53_R337L and TP53_Y220C mutations were detected only through the proposed method. The proposed method can detect mutations from blood, this detection result was consistent with the cancer tissues of the same clinical lung cancer patient.The proposed Ion-Proton technology-based NGS method can detect genetic mutations in Chinese lung cancer patients. Therefore, the proposed method could be used to detect mutations in other cancer tissues and plasma, which needs further validation." @default.
• W3039750872 created "2020-07-10" @default.
• W3039750872 creator A5011878271 @default.
• W3039750872 creator A5051153879 @default.
• W3039750872 creator A5052676364 @default.
• W3039750872 creator A5055223470 @default.
• W3039750872 creator A5069813415 @default.
• W3039750872 creator A5073250498 @default.
• W3039750872 creator A5074151953 @default.
• W3039750872 creator A5075426616 @default.
• W3039750872 creator A5083650470 @default.
• W3039750872 date "2020-07-01" @default.
• W3039750872 modified "2023-09-24" @default.
• W3039750872 title "Establishment and application of a method of next generation sequencing of 285 genes in lung cancer based on Ion-Proton platform" @default.
• W3039750872 cites W1192724814 @default.
• W3039750872 cites W1863845686 @default.
• W3039750872 cites W1980852206 @default.
• W3039750872 cites W1996641286 @default.
• W3039750872 cites W1997476230 @default.
• W3039750872 cites W2008167816 @default.
• W3039750872 cites W2030327197 @default.
• W3039750872 cites W2089135124 @default.
• W3039750872 cites W2104750097 @default.
• W3039750872 cites W2107371806 @default.
• W3039750872 cites W2119818111 @default.
• W3039750872 cites W2125204079 @default.
• W3039750872 cites W2126927831 @default.
• W3039750872 cites W2129415103 @default.
• W3039750872 cites W2136941319 @default.
• W3039750872 cites W2331681543 @default.
• W3039750872 cites W2557256009 @default.
• W3039750872 cites W2593435710 @default.
• W3039750872 cites W2765268335 @default.
• W3039750872 cites W2777183436 @default.
• W3039750872 doi "https://doi.org/10.21037/tcr-19-2855" @default.
• W3039750872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35117791" @default.
• W3039750872 hasPublicationYear "2020" @default.
• W3039750872 type Work @default.
• W3039750872 sameAs 3039750872 @default.
• W3039750872 citedByCount "0" @default.
• W3039750872 crossrefType "journal-article" @default.
• W3039750872 hasAuthorship W3039750872A5011878271 @default.
• W3039750872 hasAuthorship W3039750872A5051153879 @default.
• W3039750872 hasAuthorship W3039750872A5052676364 @default.
• W3039750872 hasAuthorship W3039750872A5055223470 @default.
• W3039750872 hasAuthorship W3039750872A5069813415 @default.
• W3039750872 hasAuthorship W3039750872A5073250498 @default.
• W3039750872 hasAuthorship W3039750872A5074151953 @default.
• W3039750872 hasAuthorship W3039750872A5075426616 @default.
• W3039750872 hasAuthorship W3039750872A5083650470 @default.
• W3039750872 hasBestOaLocation W30397508721 @default.
• W3039750872 hasConcept C104317684 @default.
• W3039750872 hasConcept C119054055 @default.
• W3039750872 hasConcept C135763542 @default.
• W3039750872 hasConcept C143998085 @default.
• W3039750872 hasConcept C153209595 @default.
• W3039750872 hasConcept C153911025 @default.
• W3039750872 hasConcept C164928082 @default.
• W3039750872 hasConcept C2776256026 @default.
• W3039750872 hasConcept C51679486 @default.
• W3039750872 hasConcept C54355233 @default.
• W3039750872 hasConcept C71924100 @default.
• W3039750872 hasConcept C76818968 @default.
• W3039750872 hasConcept C86803240 @default.
• W3039750872 hasConceptScore W3039750872C104317684 @default.
• W3039750872 hasConceptScore W3039750872C119054055 @default.
• W3039750872 hasConceptScore W3039750872C135763542 @default.
• W3039750872 hasConceptScore W3039750872C143998085 @default.
• W3039750872 hasConceptScore W3039750872C153209595 @default.
• W3039750872 hasConceptScore W3039750872C153911025 @default.
• W3039750872 hasConceptScore W3039750872C164928082 @default.
• W3039750872 hasConceptScore W3039750872C2776256026 @default.
• W3039750872 hasConceptScore W3039750872C51679486 @default.
• W3039750872 hasConceptScore W3039750872C54355233 @default.
• W3039750872 hasConceptScore W3039750872C71924100 @default.
• W3039750872 hasConceptScore W3039750872C76818968 @default.
• W3039750872 hasConceptScore W3039750872C86803240 @default.
• W3039750872 hasIssue "7" @default.
• W3039750872 hasLocation W30397508721 @default.
• W3039750872 hasLocation W30397508722 @default.
• W3039750872 hasLocation W30397508723 @default.
• W3039750872 hasOpenAccess W3039750872 @default.
• W3039750872 hasPrimaryLocation W30397508721 @default.
• W3039750872 hasRelatedWork W188686869 @default.
• W3039750872 hasRelatedWork W1915352652 @default.
• W3039750872 hasRelatedWork W2089548728 @default.
• W3039750872 hasRelatedWork W2142453541 @default.
• W3039750872 hasRelatedWork W2422550876 @default.
• W3039750872 hasRelatedWork W2589715632 @default.
• W3039750872 hasRelatedWork W2605297139 @default.
• W3039750872 hasRelatedWork W2803576017 @default.
• W3039750872 hasRelatedWork W2896982634 @default.
• W3039750872 hasRelatedWork W3023517203 @default.
• W3039750872 hasVolume "9" @default.
• W3039750872 isParatext "false" @default.
• W3039750872 isRetracted "false" @default.
• W3039750872 magId "3039750872" @default.
• W3039750872 workType "article" @default.