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W3044914928 abstract "Abstract In many species, alpha-lipoic acid (ALA) is essential for embryo development. There, therefore, was investigation of effects of ALA supplementation to culture media for in vitro development of cattle embryos. In Experiment I, there were assessments of embryo production and oxidative status of cattle embryos derived by in vitro maturation and fertilization (IVM/IVF)that were cultured until the blastocyst stage of development using different ALA concentrations (5, 25 and 100 μM), fetal bovine serum (FBS) and amino acids (aa) as well as 20 % oxygen (O2) in the culture atmosphere. In Experiment II, embryos were cultured without FBS, at different ALA concentrations (2.5, 5 and 7.5 μM) and in the presence or absence of aa when there was a 7 % O2 atmosphere. Embryo development rates and blastocyst quality were evaluated. With 20 % O2 concentration, treatment with 100 μM ALA resulted in lesser hatching rates and development to the blastocyst stage (P" @default.
W3044914928 created "2020-07-29" @default.
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W3044914928 date "2020-10-01" @default.
W3044914928 modified "2023-10-18" @default.
W3044914928 title "Effect of alpha-lipoic acid during preimplantation development of cattle embryos when there were different in vitro culture conditions" @default.
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W3044914928 doi "https://doi.org/10.1016/j.anireprosci.2020.106550" @default.
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