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- W3172612314 abstract "Mammalian retina exhibits a highly organized laminar structure consisting more than fifty cell types which are produced and eventually differentiated in specific manner during development. Several gene expression studies have been performed in retina. However, the landscape of alternatively spliced isoforms on global level at embryonic and postnatal stages of retinal development has not been done. Here we employed high throughput RNAseq from illumina HiSeq 2000 platform for retina samples of embryonic day, E16 and postnatal day, P0. The sequence reads were analyzed by using combination of tools such as Bowtie, IsoEM, Cuflinks and Seqmonk. We have identified 18021 and 17320 transcripts in E16 and P0 time points, respectively. Comparison of the E16 and P0 transcriptomes showed differential expression patterns and levels (as FPKM values) of transcripts. qRT-PCR analysis of genes such as Pax6 further confirmed the results. Our results suggest that regulation of alternative spliced isoforms may be the key to the differentiation of retinal cell-types during development. Our approach of global transcriptome characterization would advance the findings in cellular networks of proteins required for specific functions and transitions required in the developing retina. In all, this analysis will provide a database of alternatively spliced isoforms of genes expressed in the retina. Funding from NEI K99/ROO-5ROOEYO1954704." @default.
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- W3172612314 date "2013-04-01" @default.
- W3172612314 modified "2023-10-17" @default.
- W3172612314 title "RNAseq analysis of embryonic and postnatal stages of mouse retina reveal transcriptome diversity and novel alternatively spliced isoforms" @default.
- W3172612314 doi "https://doi.org/10.1096/fasebj.27.1_supplement.775.5" @default.
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