FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3197529907> ?p ?o ?g. }

• W3197529907 abstract "Results from previous investigations into spontaneous rifampin resistance (Rifr) mutations in the Bacillus subtilis rpoB gene suggested that the spectrum of mutations depends on the growth environment. However, these studies were limited by low sample numbers, allowing for the potential distortion of the data by the presence of jackpot mutations that may have arisen early in the growth of a population. Here, we addressed this issue by performing fluctuation analyses to assess both the rate and spectrum of Rifr mutations in two distinct media: LB, a complete laboratory medium, and SMMAsn, a minimal medium utilizing l-asparagine as the sole carbon source. We cultivated 60 separate populations under each growth condition and determined the mutation rate to Rifr to be slightly but significantly higher in LB cultures. We then sequenced the relevant regions of rpoB to map the spectrum of Rifr mutations under each growth condition. We found a distinct spectrum of mutations in each medium; LB cultures were dominated by the H482Y mutation (27/53 or 51%), whereas SMMAsn cultures were dominated by the S487L mutation (24/51 or 47%). Furthermore, we found through competition experiments that the relative fitness of the S487L mutant was significantly higher in SMMAsn than in LB medium. We therefore conclude that both the spectrum of Rifr mutations in the B. subtilis rpoB gene and the fitness of resulting mutants are influenced by the growth environment. IMPORTANCE The rpoB gene encodes the beta subunit of RNA polymerase, and mutations in rpoB are key determinants of resistance to the clinically important antibiotic rifampin. We show here that the spectrum of mutations in Bacillus subtilis rpoB depends on the medium in which the cells are cultivated. The results show that the growth environment not only plays a role in natural selection and fitness but also influences the probability of mutation at particular bases within the target gene." @default.
• W3197529907 created "2021-09-13" @default.
• W3197529907 creator A5000381556 @default.
• W3197529907 creator A5004846099 @default.
• W3197529907 date "2021-10-28" @default.
• W3197529907 modified "2023-09-26" @default.
• W3197529907 title "The Spectrum of Spontaneous Rifampin Resistance Mutations in the Bacillus subtilis <i>rpoB</i> Gene Depends on the Growth Environment" @default.
• W3197529907 cites W1557661275 @default.
• W3197529907 cites W1869587432 @default.
• W3197529907 cites W1878737652 @default.
• W3197529907 cites W1965274153 @default.
• W3197529907 cites W1967407306 @default.
• W3197529907 cites W1971480126 @default.
• W3197529907 cites W1986847472 @default.
• W3197529907 cites W2007980798 @default.
• W3197529907 cites W2015658684 @default.
• W3197529907 cites W2023326581 @default.
• W3197529907 cites W2026626836 @default.
• W3197529907 cites W2034254026 @default.
• W3197529907 cites W2036944766 @default.
• W3197529907 cites W2038211892 @default.
• W3197529907 cites W2046008656 @default.
• W3197529907 cites W2050339020 @default.
• W3197529907 cites W2052613794 @default.
• W3197529907 cites W2055297942 @default.
• W3197529907 cites W2057434770 @default.
• W3197529907 cites W2068372433 @default.
• W3197529907 cites W2075100243 @default.
• W3197529907 cites W2078680853 @default.
• W3197529907 cites W2080192136 @default.
• W3197529907 cites W2109206557 @default.
• W3197529907 cites W2123832578 @default.
• W3197529907 cites W2141046353 @default.
• W3197529907 cites W2142618175 @default.
• W3197529907 cites W2146746846 @default.
• W3197529907 cites W2163076177 @default.
• W3197529907 cites W2164365808 @default.
• W3197529907 cites W2174397014 @default.
• W3197529907 cites W2228739457 @default.
• W3197529907 cites W2322337465 @default.
• W3197529907 cites W2434616321 @default.
• W3197529907 cites W2584813526 @default.
• W3197529907 cites W2622934429 @default.
• W3197529907 cites W27657677 @default.
• W3197529907 cites W2791493789 @default.
• W3197529907 cites W2943079448 @default.
• W3197529907 cites W2950862496 @default.
• W3197529907 cites W2963716098 @default.
• W3197529907 cites W4229977739 @default.
• W3197529907 cites W4230628387 @default.
• W3197529907 cites W4251715317 @default.
• W3197529907 doi "https://doi.org/10.1128/aem.01237-21" @default.
• W3197529907 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8552901" @default.
• W3197529907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34495706" @default.
• W3197529907 hasPublicationYear "2021" @default.
• W3197529907 type Work @default.
• W3197529907 sameAs 3197529907 @default.
• W3197529907 citedByCount "8" @default.
• W3197529907 countsByYear W31975299072022 @default.
• W3197529907 countsByYear W31975299072023 @default.
• W3197529907 crossrefType "journal-article" @default.
• W3197529907 hasAuthorship W3197529907A5000381556 @default.
• W3197529907 hasAuthorship W3197529907A5004846099 @default.
• W3197529907 hasBestOaLocation W31975299072 @default.
• W3197529907 hasConcept C104317684 @default.
• W3197529907 hasConcept C143065580 @default.
• W3197529907 hasConcept C144024400 @default.
• W3197529907 hasConcept C149923435 @default.
• W3197529907 hasConcept C2777272437 @default.
• W3197529907 hasConcept C2778340795 @default.
• W3197529907 hasConcept C2908647359 @default.
• W3197529907 hasConcept C42062724 @default.
• W3197529907 hasConcept C501734568 @default.
• W3197529907 hasConcept C523546767 @default.
• W3197529907 hasConcept C54355233 @default.
• W3197529907 hasConcept C86803240 @default.
• W3197529907 hasConcept C98638677 @default.
• W3197529907 hasConceptScore W3197529907C104317684 @default.
• W3197529907 hasConceptScore W3197529907C143065580 @default.
• W3197529907 hasConceptScore W3197529907C144024400 @default.
• W3197529907 hasConceptScore W3197529907C149923435 @default.
• W3197529907 hasConceptScore W3197529907C2777272437 @default.
• W3197529907 hasConceptScore W3197529907C2778340795 @default.
• W3197529907 hasConceptScore W3197529907C2908647359 @default.
• W3197529907 hasConceptScore W3197529907C42062724 @default.
• W3197529907 hasConceptScore W3197529907C501734568 @default.
• W3197529907 hasConceptScore W3197529907C523546767 @default.
• W3197529907 hasConceptScore W3197529907C54355233 @default.
• W3197529907 hasConceptScore W3197529907C86803240 @default.
• W3197529907 hasConceptScore W3197529907C98638677 @default.
• W3197529907 hasFunder F4320306114 @default.
• W3197529907 hasIssue "22" @default.
• W3197529907 hasLocation W31975299071 @default.
• W3197529907 hasLocation W31975299072 @default.
• W3197529907 hasLocation W31975299073 @default.
• W3197529907 hasOpenAccess W3197529907 @default.
• W3197529907 hasPrimaryLocation W31975299071 @default.
• W3197529907 hasRelatedWork W101124211 @default.
• W3197529907 hasRelatedWork W1553707838 @default.
• W3197529907 hasRelatedWork W1838459654 @default.

• next