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- W4214881429 abstract "ABSTRACT Microtubules are generated at centrosomes, chromosomes, and within spindles during cell division. Whereas microtubule nucleation at the centrosome is well characterized, much remains unknown about where, when, and how microtubules are nucleated at chromosomes. To address these questions, we reconstituted microtubule nucleation from purified chromosomes in meiotic Xenopus egg extract and found that chromosomes alone can form spindles. We visualized microtubule nucleation at chromosomes using total internal reflection fluorescence microscopy to find that this occurs through branching microtubule nucleation. The initial branches nucleate near and towards kinetochores, helping explain how kinetochores might be efficiently captured. By depleting molecular motors, we find that the organization of the resultant polar branched networks is consistent with a theoretical model where the effectors for branching nucleation are released by chromosomes, forming a concentration gradient around them that spatially biases branching nucleation. In the presence of motors, these branched networks are organized into multipolar spindles." @default.
- W4214881429 created "2022-03-05" @default.
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- W4214881429 date "2022-03-01" @default.
- W4214881429 modified "2023-09-29" @default.
- W4214881429 title "Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes" @default.
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- W4214881429 doi "https://doi.org/10.1101/2022.02.28.482415" @default.
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