Matches in SemOpenAlex for { <https://semopenalex.org/work/W4239910063> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W4239910063 endingPage "355" @default.
- W4239910063 startingPage "354" @default.
- W4239910063 abstract "The liver cytosolic enzyme tryptophan 2,3-dioxygenase (TDO) catalyzes the oxidation of l-tryptophan to formylkynurenine and controls the physiological flux of tryptophan into both the serotonergic and kynureninic pathways. This hemoprotein enzyme is composed of four noncovalently bound subunits of equivalent mass and contains two heme moieties per molecule. Electron paramagnetic resonance analyses have indicated that a histidyl nitrogen is involved in heme ligation [Henry et al., (1976) J. Biol. Chem. 251, 1578], but the identity of the His residue(s) is unknown. In an attempt to characterize the active site of the enzyme we have substituted each of the 12 His residues in the rat TDO subunit with Ala, to determine their relative importance in heme binding. Sequence alignment of the rat liver protein with that of known or putative TDO sequences from other organisms reveals that four of the His residues are conserved in eukaryotes, two of which are also conserved in prokaryotes. Our findings indicate that replacement of the evolutionarily conserved His 76 and 328 residues resulted in a dramatic reduction of TDO activity, whereas that of the eukaryotically conserved His70 resulted in a significant reduction relative to that of the wild-type enzyme. On the other hand, replacement of the other eukaryotically conserved His273 residue, while affecting the relative expression of the enzyme, had little effect on its specific activity. Size-exclusion analyses revealed that the His76Ala and His328Ala mutants retained little or no heme, suggesting that these may be key residues in ligating the prosthetic heme moieties. Whether these His residues are both provided by the same TDO subunit or a different TDO subunit remains to be determined." @default.
- W4239910063 created "2022-05-12" @default.
- W4239910063 date "2001-08-01" @default.
- W4239910063 modified "2023-09-25" @default.
- W4239910063 title "Author Index for Volume 392" @default.
- W4239910063 doi "https://doi.org/10.1006/abbi.2001.2527" @default.
- W4239910063 hasPublicationYear "2001" @default.
- W4239910063 type Work @default.
- W4239910063 citedByCount "0" @default.
- W4239910063 crossrefType "journal-article" @default.
- W4239910063 hasConcept C104317684 @default.
- W4239910063 hasConcept C167625842 @default.
- W4239910063 hasConcept C181199279 @default.
- W4239910063 hasConcept C185592680 @default.
- W4239910063 hasConcept C199216141 @default.
- W4239910063 hasConcept C20705724 @default.
- W4239910063 hasConcept C2776217839 @default.
- W4239910063 hasConcept C2776706248 @default.
- W4239910063 hasConcept C2779856020 @default.
- W4239910063 hasConcept C2781338088 @default.
- W4239910063 hasConcept C515207424 @default.
- W4239910063 hasConcept C55493867 @default.
- W4239910063 hasConcept C71240020 @default.
- W4239910063 hasConceptScore W4239910063C104317684 @default.
- W4239910063 hasConceptScore W4239910063C167625842 @default.
- W4239910063 hasConceptScore W4239910063C181199279 @default.
- W4239910063 hasConceptScore W4239910063C185592680 @default.
- W4239910063 hasConceptScore W4239910063C199216141 @default.
- W4239910063 hasConceptScore W4239910063C20705724 @default.
- W4239910063 hasConceptScore W4239910063C2776217839 @default.
- W4239910063 hasConceptScore W4239910063C2776706248 @default.
- W4239910063 hasConceptScore W4239910063C2779856020 @default.
- W4239910063 hasConceptScore W4239910063C2781338088 @default.
- W4239910063 hasConceptScore W4239910063C515207424 @default.
- W4239910063 hasConceptScore W4239910063C55493867 @default.
- W4239910063 hasConceptScore W4239910063C71240020 @default.
- W4239910063 hasIssue "2" @default.
- W4239910063 hasLocation W42399100631 @default.
- W4239910063 hasOpenAccess W4239910063 @default.
- W4239910063 hasPrimaryLocation W42399100631 @default.
- W4239910063 hasRelatedWork W1508245565 @default.
- W4239910063 hasRelatedWork W1967372919 @default.
- W4239910063 hasRelatedWork W2012506760 @default.
- W4239910063 hasRelatedWork W2016750393 @default.
- W4239910063 hasRelatedWork W2037640838 @default.
- W4239910063 hasRelatedWork W2056670511 @default.
- W4239910063 hasRelatedWork W2078619688 @default.
- W4239910063 hasRelatedWork W2117640109 @default.
- W4239910063 hasRelatedWork W2359094391 @default.
- W4239910063 hasRelatedWork W958152341 @default.
- W4239910063 hasVolume "392" @default.
- W4239910063 isParatext "false" @default.
- W4239910063 isRetracted "false" @default.
- W4239910063 workType "article" @default.