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- W4298363643 abstract "To study the development of alloreactive cytolytic T cells in vivo, C57BL/6 mice were implanted s.c. with polyurethane sponges bearing allogeneic (DBA/2) splenocytes. On various days thereafter, cells that had accumulated in these sponge grafts were tested for cytolytic activity against DBA/2 target cells in 51Cr-release assays, and for frequency of DBA/2-reactive CTL as determined by limiting dilution analysis (LDA). During these studies we found that LDA was consistently more efficient at detecting alloreactive CTL than the traditional 51Cr-release assays. As determined by LDA, sponge grafts initially infused with DBA/2 splenocytes acquired high levels of DBA/2-reactive CTL, while sponge grafts infused only with saline acquired few." @default.
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- W4298363643 date "1986-01-01" @default.
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- W4298363643 title "IN VIVO MECHANISMS OF ALLOREACTIVITY" @default.
- W4298363643 doi "https://doi.org/10.1097/00007890-198601000-00016" @default.
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