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- W4313356726 abstract "Abstract New fluorophores offered by BD Biosciences, BD HorizonTM BV421 and BD HorizonTM BV480, have unique spectra, are very bright, photostable, and work well in immunofluorescence microscopy. With narrow excitation and emission spectra these 2 fluorophores are compatible with most fluorescent protein models (eGFP, YFP, RFP, and tdTomato). Additionally, they are compatible with the standard green, yellow, red, and far-red immunofluorescence imaging reagents, (Alexa Fluor® 488, Alexa Fluor® 568, and Alexa Fluor® 647). These new fluorophores enable robust 5-color imaging. The workflow is easy and the dyes are functional (with simple optimization) on most fluorescence microscopes. No spectral unmixing is required for analysis. Because BV421 and BV480 are brighter than standard imaging fluorophores, there is the potential to lower the limit of detection with improved resolution. Side by side experiments with standard fluorophores on an epifluorescence microscope demonstrate improved resolution, via increased signal to noise, using both BV421 and BV480. Brighter fluorophores improve ease of use during microscope focusing and data acquisition by decreasing exposure time. In addition to enhanced sensitivity, these fluorophores enable easy and routine 5 color staining. Examples from human and mouse model systems using directly conjugated primary antibodies, show how these dyes enable simple multi-parameter imaging thereby permitting correlation of more parameters per sample" @default.
- W4313356726 created "2023-01-06" @default.
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- W4313356726 date "2015-05-01" @default.
- W4313356726 modified "2023-09-26" @default.
- W4313356726 title "Incorporation of BD HorizonTM BV421 and BD HorizonTM BV480 immunofluorescence reagents improves immunofluorescence microscopy (TECH3P.933)" @default.
- W4313356726 doi "https://doi.org/10.4049/jimmunol.194.supp.207.3" @default.
- W4313356726 hasPublicationYear "2015" @default.
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