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- W4313384401 abstract "Abstract Analysis of lymphocytes in bulk masks the heterogeneity of cellular processes. Through advancements in single cell technologies finer resolution and the true complexities of cell populations can be revealed. A major limitation of single cell technologies, especially those that enable high-throughput screening is the poor survival and proliferation of individual cells when binned separately in vitro. We have developed a novel platform technology, termed microbubbles which are scalable arrays of individual nanoliter size wells of unique spherical architecture and gas exchange properties that enable single cells to rapidly condition their microenvironment and enable long-term culture. Additionally, morphological heterogeneity is maintained in the microbubbles, unlike in conventional tissue culture wells. Microbubble arrays are compatible with conventional fluorescent microscopes and morphology and cell surface protein expression can be easily monitored longitudinally. Secreted cellular products such as antibodies and cytokines accumulate and are readily detected from cells in real-time using fluorescent probes. Arrays with 100,000 microbubbles, contained within a footprint of a standard microscope slide, can be screened using custom software in brightfield and three fluorescent channels within 10 minutes, and rare cells of interest identified based on morphological and/or functional properties are easily recovered for downstream analysis. Applications of microbubbles developed thus far include identification and isolation of rare antibody secreting cells based on antibody-specificity for monoclonal antibody development, monitoring T cell cytokine production, and characterizing cancer stem cells." @default.
- W4313384401 created "2023-01-06" @default.
- W4313384401 creator A5046827479 @default.
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- W4313384401 date "2017-05-01" @default.
- W4313384401 modified "2023-09-27" @default.
- W4313384401 title "Microbubbles: A high-throughput platform for the sustained culture, characterization, and isolation of single cells based on morphology, cytokine production, and antibody secretion." @default.
- W4313384401 doi "https://doi.org/10.4049/jimmunol.198.supp.81.1" @default.
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