FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4319984893> ?p ?o ?g. }

• W4319984893 endingPage "1684" @default.
• W4319984893 startingPage "1684" @default.
• W4319984893 abstract "Immunogenicity is a major issue associated with the PK, efficacy, and safety evaluation of therapeutic protein products during pre-clinical and clinical studies. A multi-tiered approach consisting of screening, confirmatory, and titration assays has been widely adopted for anti-drug antibody testing. GQ1001, a recombinant humanized anti-human epidermal growth factor receptor 2 monoclonal antibody covalently linked to a cytotoxin of DM1, possesses a novel format of antibody-drug conjugates. In this study, we reported the development, validation, and application of an acid-dissociation bridging enzyme-linked immunosorbent assay for the detection of antibodies against GQ1001 in cynomolgus monkey serum. The sensitivity of the screening assay was 126.141 ng/mL in undiluted serum. The screening assay and confirmatory assay were neither affected by the naïve monkey serum nor by 2% and 5% (v/v) erythrocyte hemolysates. Moreover, the assay was not subject to interference by 2500 ng/mL of human IgG1 in the samples. Drug interference at low positive control (150 ng/mL) and high positive control (8000 ng/mL) of anti-GQ1001 antibodies was not observed when GQ1001 concentrations were below 3.125 μg/mL and 100 μg/mL, respectively. Furthermore, no hook effect was observed for the positive antibodies in the concentration range of 8 to 64 μg/mL. The validated assay was, thereafter, successfully applied to a single-dose toxicity study of GQ1001. Anti-drug antibody positive rates among dosing animals and testing samples were reported, and no significant impact was found on toxicokinetic outcomes." @default.
• W4319984893 created "2023-02-11" @default.
• W4319984893 creator A5008456036 @default.
• W4319984893 creator A5020364531 @default.
• W4319984893 creator A5025558205 @default.
• W4319984893 creator A5027030871 @default.
• W4319984893 creator A5037234869 @default.
• W4319984893 creator A5037882273 @default.
• W4319984893 creator A5046742733 @default.
• W4319984893 creator A5048514285 @default.
• W4319984893 creator A5048776297 @default.
• W4319984893 creator A5060978933 @default.
• W4319984893 creator A5065723135 @default.
• W4319984893 creator A5076863688 @default.
• W4319984893 creator A5082638187 @default.
• W4319984893 date "2023-02-10" @default.
• W4319984893 modified "2023-09-26" @default.
• W4319984893 title "Development, Validation and Application of a Bridging ELISA for Detection of Antibodies against GQ1001 in Cynomolgus Monkey Serum" @default.
• W4319984893 cites W1997496599 @default.
• W4319984893 cites W2009032064 @default.
• W4319984893 cites W2050818535 @default.
• W4319984893 cites W2064839484 @default.
• W4319984893 cites W2073248711 @default.
• W4319984893 cites W2079240335 @default.
• W4319984893 cites W2253966500 @default.
• W4319984893 cites W2597045666 @default.
• W4319984893 cites W2771355213 @default.
• W4319984893 cites W2903196646 @default.
• W4319984893 cites W2935912602 @default.
• W4319984893 cites W2950827632 @default.
• W4319984893 cites W3047170482 @default.
• W4319984893 cites W3128485166 @default.
• W4319984893 cites W3137566061 @default.
• W4319984893 cites W3159684865 @default.
• W4319984893 cites W3187784120 @default.
• W4319984893 cites W4220847494 @default.
• W4319984893 cites W4297236733 @default.
• W4319984893 doi "https://doi.org/10.3390/molecules28041684" @default.
• W4319984893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36838672" @default.
• W4319984893 hasPublicationYear "2023" @default.
• W4319984893 type Work @default.
• W4319984893 citedByCount "0" @default.
• W4319984893 crossrefType "journal-article" @default.
• W4319984893 hasAuthorship W4319984893A5008456036 @default.
• W4319984893 hasAuthorship W4319984893A5020364531 @default.
• W4319984893 hasAuthorship W4319984893A5025558205 @default.
• W4319984893 hasAuthorship W4319984893A5027030871 @default.
• W4319984893 hasAuthorship W4319984893A5037234869 @default.
• W4319984893 hasAuthorship W4319984893A5037882273 @default.
• W4319984893 hasAuthorship W4319984893A5046742733 @default.
• W4319984893 hasAuthorship W4319984893A5048514285 @default.
• W4319984893 hasAuthorship W4319984893A5048776297 @default.
• W4319984893 hasAuthorship W4319984893A5060978933 @default.
• W4319984893 hasAuthorship W4319984893A5065723135 @default.
• W4319984893 hasAuthorship W4319984893A5076863688 @default.
• W4319984893 hasAuthorship W4319984893A5082638187 @default.
• W4319984893 hasBestOaLocation W43199848931 @default.
• W4319984893 hasConcept C104317684 @default.
• W4319984893 hasConcept C153911025 @default.
• W4319984893 hasConcept C159654299 @default.
• W4319984893 hasConcept C185592680 @default.
• W4319984893 hasConcept C203014093 @default.
• W4319984893 hasConcept C2780420688 @default.
• W4319984893 hasConcept C2780868878 @default.
• W4319984893 hasConcept C40767141 @default.
• W4319984893 hasConcept C43617362 @default.
• W4319984893 hasConcept C542903549 @default.
• W4319984893 hasConcept C55493867 @default.
• W4319984893 hasConcept C71924100 @default.
• W4319984893 hasConcept C86803240 @default.
• W4319984893 hasConcept C98274493 @default.
• W4319984893 hasConceptScore W4319984893C104317684 @default.
• W4319984893 hasConceptScore W4319984893C153911025 @default.
• W4319984893 hasConceptScore W4319984893C159654299 @default.
• W4319984893 hasConceptScore W4319984893C185592680 @default.
• W4319984893 hasConceptScore W4319984893C203014093 @default.
• W4319984893 hasConceptScore W4319984893C2780420688 @default.
• W4319984893 hasConceptScore W4319984893C2780868878 @default.
• W4319984893 hasConceptScore W4319984893C40767141 @default.
• W4319984893 hasConceptScore W4319984893C43617362 @default.
• W4319984893 hasConceptScore W4319984893C542903549 @default.
• W4319984893 hasConceptScore W4319984893C55493867 @default.
• W4319984893 hasConceptScore W4319984893C71924100 @default.
• W4319984893 hasConceptScore W4319984893C86803240 @default.
• W4319984893 hasConceptScore W4319984893C98274493 @default.
• W4319984893 hasIssue "4" @default.
• W4319984893 hasLocation W43199848931 @default.
• W4319984893 hasLocation W43199848932 @default.
• W4319984893 hasLocation W43199848933 @default.
• W4319984893 hasOpenAccess W4319984893 @default.
• W4319984893 hasPrimaryLocation W43199848931 @default.
• W4319984893 hasRelatedWork W1591820460 @default.
• W4319984893 hasRelatedWork W1899411185 @default.
• W4319984893 hasRelatedWork W195682249 @default.
• W4319984893 hasRelatedWork W2343297415 @default.
• W4319984893 hasRelatedWork W2368460920 @default.
• W4319984893 hasRelatedWork W2380360044 @default.
• W4319984893 hasRelatedWork W2380817849 @default.

• next