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- W4378840003 abstract "(Biophysical Journal 122, 1720–1731; May 2, 2023) In Figure 2F, the scale bar was incorrectly labeled as “100 μm”; it should read “20 μm“ instead. A corrected version of the figure appears below.Figure 2 (A and B) Rectangular microfluidic channels 300 μm in width and 100 μm in height were plasma-bonded to a glass coverslip. (C) Fluorescent membrane patches (red) overlaid with a bright-field image showing the edges of the microfluidic channel. (D–F) A single membrane patch labeled with both TXRed-DPPE (red, top row) and with fluorescent neutravidin (green, bottom row) shown before flow (left), during flow (middle), and 21 min after flow stopped (right). In all images, flow direction was from right to left. To see this figure in color, go online (original).View Large Image Figure ViewerDownload Hi-res image Download (PPT) Measuring flow-mediated protein drift across stationary supported lipid bilayersRatajczak et al.Biophysical JournalApril 4, 2023In BriefFluid flow near biological membranes influences cell functions such as development, motility, and environmental sensing. Flow can laterally transport extracellular membrane proteins located at the cell-fluid interface. To determine whether this transport contributes to flow signaling in cells, quantitative knowledge of the forces acting on membrane proteins is required. Here, we demonstrate a method for measuring flow-mediated lateral transport of lipid-anchored proteins. We rupture giant unilamellar vesicles to form discrete patches of supported membrane inside rectangular microchannels and then allow proteins to bind to the upper surface of the membrane. Full-Text PDF Open Access" @default.
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- W4378840003 date "2023-06-01" @default.
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- W4378840003 title "Measuring flow-mediated protein drift across stationary supported lipid bilayers" @default.
- W4378840003 doi "https://doi.org/10.1016/j.bpj.2023.05.031" @default.
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