Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379880510> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W4379880510 abstract "The pathogenesis of severe Plasmodium falciparum malaria involves cytoadhesive microvascular sequestration of infected erythrocytes, mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1). PfEMP1 variants are encoded by the highly polymorphic family of var genes, the sequences of which are largely unknown in clinical samples. Previously, we published new approaches for var gene profiling and classification of predicted binding phenotypes in clinical P. falciparum isolates (), which represented a major technical advance. Building on this, we report here a novel method for var gene assembly and multidimensional quantification from RNA-sequencing that even outperforms the earlier approach of on both laboratory and clinical isolates across a combination of metrics. It is a powerful tool to interrogate the var transcriptome in context with the rest of the transcriptome and can be applied to enhance our understanding of the role of var genes in malaria pathogenesis. We applied this new method to investigate changes in var gene expression through early transition to in vitro culture, using paired sets of ex vivo samples from our previous study, cultured for up to three generations. In parallel, changes in non-polymorphic core gene expression were investigated. Unpredictable var gene switching and convergence towards var2csa were observed in culture, along with differential expression of 19% of the core transcriptome between paired ex vivo and generation 1 samples. Our results cast doubt on the validity of the common practice of using short-term cultured parasites to make inferences about in vivo phenotype and behaviour." @default.
- W4379880510 created "2023-06-09" @default.
- W4379880510 date "2023-06-08" @default.
- W4379880510 modified "2023-10-01" @default.
- W4379880510 title "Reviewer #2 (Public Review): A novel computational pipeline for var gene expression augments the discovery of changes in the Plasmodium falciparum transcriptome during transition from in vivo to short-term in vitro culture" @default.
- W4379880510 doi "https://doi.org/10.7554/elife.87726.1.sa2" @default.
- W4379880510 hasPublicationYear "2023" @default.
- W4379880510 type Work @default.
- W4379880510 citedByCount "0" @default.
- W4379880510 crossrefType "peer-review" @default.
- W4379880510 hasBestOaLocation W43798805101 @default.
- W4379880510 hasConcept C104317684 @default.
- W4379880510 hasConcept C107397762 @default.
- W4379880510 hasConcept C127716648 @default.
- W4379880510 hasConcept C150194340 @default.
- W4379880510 hasConcept C151730666 @default.
- W4379880510 hasConcept C162317418 @default.
- W4379880510 hasConcept C18431079 @default.
- W4379880510 hasConcept C203014093 @default.
- W4379880510 hasConcept C207001950 @default.
- W4379880510 hasConcept C2778048844 @default.
- W4379880510 hasConcept C2778371730 @default.
- W4379880510 hasConcept C2779343474 @default.
- W4379880510 hasConcept C54355233 @default.
- W4379880510 hasConcept C70721500 @default.
- W4379880510 hasConcept C86803240 @default.
- W4379880510 hasConceptScore W4379880510C104317684 @default.
- W4379880510 hasConceptScore W4379880510C107397762 @default.
- W4379880510 hasConceptScore W4379880510C127716648 @default.
- W4379880510 hasConceptScore W4379880510C150194340 @default.
- W4379880510 hasConceptScore W4379880510C151730666 @default.
- W4379880510 hasConceptScore W4379880510C162317418 @default.
- W4379880510 hasConceptScore W4379880510C18431079 @default.
- W4379880510 hasConceptScore W4379880510C203014093 @default.
- W4379880510 hasConceptScore W4379880510C207001950 @default.
- W4379880510 hasConceptScore W4379880510C2778048844 @default.
- W4379880510 hasConceptScore W4379880510C2778371730 @default.
- W4379880510 hasConceptScore W4379880510C2779343474 @default.
- W4379880510 hasConceptScore W4379880510C54355233 @default.
- W4379880510 hasConceptScore W4379880510C70721500 @default.
- W4379880510 hasConceptScore W4379880510C86803240 @default.
- W4379880510 hasLocation W43798805101 @default.
- W4379880510 hasOpenAccess W4379880510 @default.
- W4379880510 hasPrimaryLocation W43798805101 @default.
- W4379880510 hasRelatedWork W2563598809 @default.
- W4379880510 hasRelatedWork W2590201838 @default.
- W4379880510 hasRelatedWork W2734403094 @default.
- W4379880510 hasRelatedWork W2782502720 @default.
- W4379880510 hasRelatedWork W2991522968 @default.
- W4379880510 hasRelatedWork W3005714460 @default.
- W4379880510 hasRelatedWork W3012091774 @default.
- W4379880510 hasRelatedWork W4224439911 @default.
- W4379880510 hasRelatedWork W4379875417 @default.
- W4379880510 hasRelatedWork W4379880600 @default.
- W4379880510 isParatext "false" @default.
- W4379880510 isRetracted "false" @default.
- W4379880510 workType "peer-review" @default.