Matches in SemOpenAlex for { <https://semopenalex.org/work/W4379929135> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4379929135 endingPage "134109" @default.
- W4379929135 startingPage "134109" @default.
- W4379929135 abstract "Droplet digital polymerase chain reaction (ddPCR) is an extremely sensitive method for the precisely determining the concentration of target nucleic acids. However, air bubbles between droplets during amplification can cause significant droplet loss and decreased accuracy in results. In the present study, an all-in-one microfluidic chip that integrates emulsification, passive bubble removal, droplet monolayer storage, on-chip nucleic acid amplification, and droplet fluorescence signal readout is proposed. The integrated passive bubble removal structures automatically complete the trapping and guiding of the bubbles, ensuring that the droplets do not touch the bubbles during amplification and thus is not lost. The ddPCR device with optimized key parameters proved to be effective and efficient by completely removing bubbles between droplets and having a dead volume of less than 1 %. The ability of the ddPCR chip to accurately quantify nucleic acids was evaluated by measuring plasmids with the SARS-CoV-2N gene at concentrations ranging from 10 to 50 000 copies/μL. The innovative ddPCR device satisfies the requirement for accurate nucleic acid quantification and is expected to accelerate the popularity of dPCR due to its low processing difficulty, ease of use and high robustness." @default.
- W4379929135 created "2023-06-09" @default.
- W4379929135 creator A5012043525 @default.
- W4379929135 creator A5040205022 @default.
- W4379929135 creator A5046164682 @default.
- W4379929135 creator A5053613555 @default.
- W4379929135 creator A5067158080 @default.
- W4379929135 creator A5073146936 @default.
- W4379929135 creator A5087563808 @default.
- W4379929135 date "2023-10-01" @default.
- W4379929135 modified "2023-10-06" @default.
- W4379929135 title "A droplet digital PCR chip with passive bubble removal for absolute nucleic acid quantification" @default.
- W4379929135 cites W1025868516 @default.
- W4379929135 cites W1147404897 @default.
- W4379929135 cites W1988955440 @default.
- W4379929135 cites W2065099108 @default.
- W4379929135 cites W2069797500 @default.
- W4379929135 cites W2093403490 @default.
- W4379929135 cites W2119660355 @default.
- W4379929135 cites W2123621585 @default.
- W4379929135 cites W2507327823 @default.
- W4379929135 cites W2546914187 @default.
- W4379929135 cites W2547357254 @default.
- W4379929135 cites W2548144690 @default.
- W4379929135 cites W2581756571 @default.
- W4379929135 cites W2915163367 @default.
- W4379929135 cites W3010698037 @default.
- W4379929135 cites W3048282195 @default.
- W4379929135 cites W3194899284 @default.
- W4379929135 cites W4214499059 @default.
- W4379929135 cites W4280509809 @default.
- W4379929135 cites W4280638177 @default.
- W4379929135 doi "https://doi.org/10.1016/j.snb.2023.134109" @default.
- W4379929135 hasPublicationYear "2023" @default.
- W4379929135 type Work @default.
- W4379929135 citedByCount "1" @default.
- W4379929135 crossrefType "journal-article" @default.
- W4379929135 hasAuthorship W4379929135A5012043525 @default.
- W4379929135 hasAuthorship W4379929135A5040205022 @default.
- W4379929135 hasAuthorship W4379929135A5046164682 @default.
- W4379929135 hasAuthorship W4379929135A5053613555 @default.
- W4379929135 hasAuthorship W4379929135A5067158080 @default.
- W4379929135 hasAuthorship W4379929135A5073146936 @default.
- W4379929135 hasAuthorship W4379929135A5087563808 @default.
- W4379929135 hasConcept C104317684 @default.
- W4379929135 hasConcept C126307556 @default.
- W4379929135 hasConcept C157915830 @default.
- W4379929135 hasConcept C165005293 @default.
- W4379929135 hasConcept C171250308 @default.
- W4379929135 hasConcept C173608175 @default.
- W4379929135 hasConcept C185592680 @default.
- W4379929135 hasConcept C186060115 @default.
- W4379929135 hasConcept C192562407 @default.
- W4379929135 hasConcept C24107716 @default.
- W4379929135 hasConcept C41008148 @default.
- W4379929135 hasConcept C48126324 @default.
- W4379929135 hasConcept C49105822 @default.
- W4379929135 hasConcept C55493867 @default.
- W4379929135 hasConcept C76155785 @default.
- W4379929135 hasConcept C8673954 @default.
- W4379929135 hasConcept C86803240 @default.
- W4379929135 hasConceptScore W4379929135C104317684 @default.
- W4379929135 hasConceptScore W4379929135C126307556 @default.
- W4379929135 hasConceptScore W4379929135C157915830 @default.
- W4379929135 hasConceptScore W4379929135C165005293 @default.
- W4379929135 hasConceptScore W4379929135C171250308 @default.
- W4379929135 hasConceptScore W4379929135C173608175 @default.
- W4379929135 hasConceptScore W4379929135C185592680 @default.
- W4379929135 hasConceptScore W4379929135C186060115 @default.
- W4379929135 hasConceptScore W4379929135C192562407 @default.
- W4379929135 hasConceptScore W4379929135C24107716 @default.
- W4379929135 hasConceptScore W4379929135C41008148 @default.
- W4379929135 hasConceptScore W4379929135C48126324 @default.
- W4379929135 hasConceptScore W4379929135C49105822 @default.
- W4379929135 hasConceptScore W4379929135C55493867 @default.
- W4379929135 hasConceptScore W4379929135C76155785 @default.
- W4379929135 hasConceptScore W4379929135C8673954 @default.
- W4379929135 hasConceptScore W4379929135C86803240 @default.
- W4379929135 hasLocation W43799291351 @default.
- W4379929135 hasOpenAccess W4379929135 @default.
- W4379929135 hasPrimaryLocation W43799291351 @default.
- W4379929135 hasRelatedWork W2253893353 @default.
- W4379929135 hasRelatedWork W2294320876 @default.
- W4379929135 hasRelatedWork W2345507872 @default.
- W4379929135 hasRelatedWork W2562142047 @default.
- W4379929135 hasRelatedWork W2604203368 @default.
- W4379929135 hasRelatedWork W2912140360 @default.
- W4379929135 hasRelatedWork W3030469736 @default.
- W4379929135 hasRelatedWork W3179198623 @default.
- W4379929135 hasRelatedWork W3200241932 @default.
- W4379929135 hasRelatedWork W4214532968 @default.
- W4379929135 hasVolume "392" @default.
- W4379929135 isParatext "false" @default.
- W4379929135 isRetracted "false" @default.
- W4379929135 workType "article" @default.