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- W96610581 abstract "A technique for the detection of Pseudomonas syringae pv. tomato, the causal agent of bacteria speck disease, on tomato seeds is described. The method is based on the enrichment of the pathogen by adding of the seeds under test to nutrient broth for 1–3 days at 28 °C. The seeds can be used single or in lots (5, 10, 50 or 100 ones). For identification a small amount of the broth is to transmit to agar plates, especially to King's Agar B, or to inoculate in young tomato seedlings. On agar plates suspectable colonies (small, convex, hyaline, fluorescent) must be exactly identified by a specific antiserum (agglutination test). In bioassay the causal agent produces typical symptoms on the leaves. In three years lasting tests including 6,000 tomato trade seeds only in the sample of 1980 Ps. syringae pv. tomato could be detected (until to the May of the following year). The addition of selective substances (penicillin and actidione) to the enrichment broth considerably improved the detection and identifying of the typical colonies of Ps. syringae pv. tomato on agar plates. However, the method of enriching in normal nutrient broth and transmitting on the selective medium recommended by Bashan et al. (1981) is also very effective. Es wird ein Verfahren zum Nachweis von Pseudomonas syringae pv. tomato, dem Erreger der Bakteriellen Blatt- und Fruchtfleckenkrankheit, an Tomatensaatgut beschrieben. Die Methode besteht darin, daß die zu untersuchenden Samen einzeln oder in Form von Sammelproben (5, 10, 50 oder 100 Korn) für 1–3 Tage in Nährbouillon I bei 28 °C vorbebrütet werden. Aus diesem Anreicherungssubstrat werden für den Erregernachweis Flüssigkeitsproben entnommen und entweder auf einem geeigneten Agarmedium, am besten King's Agar B, geimpft oder zur Inokulation von Tomatenjungpflanzen verwendet. Beim Nachweis auf Agarplatten müssen verdächtige (kleine, konvexe, hyaline, fluoreszierende) Kolonien mit Hilfe eines spezifischen Antiserums genau identifiziert werden, während der Biotest an Tomatenpflanzen bei Vorhandensein des Erregers typische Symptome liefert. Mehrjährige durchgeführte Erhebungen an 6000 Tomatensamen aus der Praxis ergaben nur bei Saatgut aus dem Jahre 1980 einen positiven Erregernachweis (bis Mai des Folgejahres). Der Zusatz von selektiv wirksamen Hemmstoffen (Penizillin, Aktidion) zur Anreicherungsbouillon erleichtert die Erkennung und den Nachweis der typischen Ps. syringae pv. tomato-Kolonien auf Agarplatten erheblich. Sehr wirksam ist aber auch die Anreicherung auf Nährbouillon I und das Ausplatten auf dem von Bashan et al. 1981 empfohlenen Selektivsubstrat." @default.
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- W96610581 date "1986-01-01" @default.
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- W96610581 title "Zum Nachweis des Erregers der Bakteriellen Blatt- und Fruchtfleckenkrankheit, Pseudomonas syringae pv. tomato (Okabe) young, dye & wilkie, am tomatensaatgut" @default.
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- W96610581 doi "https://doi.org/10.1016/s0232-4393(86)80048-8" @default.
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