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• W1504719529 abstract "Pavlova lutheri, a marine microalga, is rich in the very long chain polyunsaturated fatty acids (VLCPUFAs) eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Using an expressed sequence tag approach, we isolated a cDNA designated Pldes1, and encoding an amino acid sequence showing high similarity with polyunsaturated fatty acid front-end desaturases. Heterologous expression in yeast demonstrated that PlDES1 desaturated 22:5n-3 and 22:4n-6 into 22:6n-3 and 22:5n-6 respectively, and was equally active on both substrates. Thus, PlDES1 is a novel VLCPUFA Delta4-desaturase. Pldes1 expression is four-fold higher during the mid-exponential phase of growth compared to late exponential and stationary phases." @default.
• W1504719529 created "2016-06-24" @default.
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• W1504719529 date "2003-10-02" @default.
• W1504719529 modified "2023-10-17" @default.
• W1504719529 title "Identification of a very long chain polyunsaturated fatty acid Δ4‐desaturase from the microalga <i>Pavlova lutheri</i>¹" @default.
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• W1504719529 doi "https://doi.org/10.1016/s0014-5793(03)01078-0" @default.
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