FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1753017813> ?p ?o ?g. }

• W1753017813 endingPage "840" @default.
• W1753017813 startingPage "826" @default.
• W1753017813 abstract "Successful therapeutic protein production in vitro and in vivo requires efficient and long-term transgene expression supported by optimized vector and transgene cis-regulatory sequence elements. This study provides a comparative analysis of CpG-rich, highly expressed, versus CpG-depleted, poorly expressed green fluorescent protein (GFP) reporter transgenes, transcribed by various promoters in two different cell systems. Long-term GFP expression from a defined locus in stable Chinese hamster ovary cells was clearly influenced by the combination of transgene CpG content and promoter usage, as shown by differential silencing effects on selection pressure removal among the cytomegalovirus (CMV) promoter and elongation factor (EF)-1α promoter. Whereas a high intragenic CpG content promoted local DNA methylation, CpG depletion rather accelerated transgene loss and increased the local chromatin density. On lentiviral transfer of various expression modules into epigenetically sensitive P19 embryonic pluripotent carcinoma cells, CMV promoter usage led to rapid gene silencing irrespective of the intragenic CpG content. In contrast, EF-1α promoter-controlled constructs showed delayed silencing activity and high-level transgene expression, in particular when the CpG-rich GFP reporter was used. Notably, GFP silencing in P19 cells could be prevented completely by the bidirectional, dual divergently transcribed A2UCOE (ubiquitously acting chromatin-opening element derived from the human HNRPA2B1-CBX3 locus) promoter. Because the level of GFP expression by the A2UCOE promoter was entirely unaffected by the intragenic CpG level, we suggest that A2UCOE can overcome chromatin compaction resulting from intragenic CpG depletion due to its ascribed chromatin-opening abilities. Our analyses provide insights into the interplay of the intragenic CpG content with promoter sequences and regulatory sequence elements, thus contributing toward the design of therapeutic transgene expression cassettes for future gene therapy applications." @default.
• W1753017813 created "2016-06-24" @default.
• W1753017813 creator A5009018699 @default.
• W1753017813 creator A5020871813 @default.
• W1753017813 creator A5038015992 @default.
• W1753017813 creator A5078107525 @default.
• W1753017813 date "2015-12-01" @default.
• W1753017813 modified "2023-09-27" @default.
• W1753017813 title "Interplay of Promoter Usage and Intragenic CpG Content: Impact on GFP Reporter Gene Expression" @default.
• W1753017813 cites W1490724066 @default.
• W1753017813 cites W1760112062 @default.
• W1753017813 cites W1969022737 @default.
• W1753017813 cites W1972817327 @default.
• W1753017813 cites W1976626368 @default.
• W1753017813 cites W1978094899 @default.
• W1753017813 cites W1979273971 @default.
• W1753017813 cites W1979311891 @default.
• W1753017813 cites W1982516399 @default.
• W1753017813 cites W1982609556 @default.
• W1753017813 cites W1982650546 @default.
• W1753017813 cites W1984554412 @default.
• W1753017813 cites W1984680322 @default.
• W1753017813 cites W1989241605 @default.
• W1753017813 cites W1992703607 @default.
• W1753017813 cites W1994390268 @default.
• W1753017813 cites W1996310432 @default.
• W1753017813 cites W2000560123 @default.
• W1753017813 cites W2005844368 @default.
• W1753017813 cites W2006846934 @default.
• W1753017813 cites W2009100845 @default.
• W1753017813 cites W2009761723 @default.
• W1753017813 cites W2010303814 @default.
• W1753017813 cites W2010655741 @default.
• W1753017813 cites W2016416222 @default.
• W1753017813 cites W2019875162 @default.
• W1753017813 cites W2021693767 @default.
• W1753017813 cites W2032719252 @default.
• W1753017813 cites W2041797639 @default.
• W1753017813 cites W2043509272 @default.
• W1753017813 cites W2044125630 @default.
• W1753017813 cites W2048220786 @default.
• W1753017813 cites W2055034240 @default.
• W1753017813 cites W2055290765 @default.
• W1753017813 cites W2056291521 @default.
• W1753017813 cites W2058895038 @default.
• W1753017813 cites W2061738853 @default.
• W1753017813 cites W2079641300 @default.
• W1753017813 cites W2091602444 @default.
• W1753017813 cites W2093760833 @default.
• W1753017813 cites W2094851045 @default.
• W1753017813 cites W2095321122 @default.
• W1753017813 cites W2099701177 @default.
• W1753017813 cites W2107983332 @default.
• W1753017813 cites W2110276409 @default.
• W1753017813 cites W2113446817 @default.
• W1753017813 cites W2116150154 @default.
• W1753017813 cites W2119324304 @default.
• W1753017813 cites W2119479524 @default.
• W1753017813 cites W2122003649 @default.
• W1753017813 cites W2124049725 @default.
• W1753017813 cites W2128593286 @default.
• W1753017813 cites W2130919037 @default.
• W1753017813 cites W2137505881 @default.
• W1753017813 cites W2138891173 @default.
• W1753017813 cites W2140651822 @default.
• W1753017813 cites W2143729842 @default.
• W1753017813 cites W2145668963 @default.
• W1753017813 cites W2146179684 @default.
• W1753017813 cites W2146809599 @default.
• W1753017813 cites W2151609707 @default.
• W1753017813 cites W2157896232 @default.
• W1753017813 cites W2163596424 @default.
• W1753017813 cites W2166933617 @default.
• W1753017813 cites W2167121947 @default.
• W1753017813 cites W2167523976 @default.
• W1753017813 cites W2182271219 @default.
• W1753017813 cites W2233541102 @default.
• W1753017813 cites W2419098194 @default.
• W1753017813 cites W4232169750 @default.
• W1753017813 doi "https://doi.org/10.1089/hum.2015.075" @default.
• W1753017813 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26414116" @default.
• W1753017813 hasPublicationYear "2015" @default.
• W1753017813 type Work @default.
• W1753017813 sameAs 1753017813 @default.
• W1753017813 citedByCount "11" @default.
• W1753017813 countsByYear W17530178132017 @default.
• W1753017813 countsByYear W17530178132018 @default.
• W1753017813 countsByYear W17530178132019 @default.
• W1753017813 countsByYear W17530178132020 @default.
• W1753017813 countsByYear W17530178132021 @default.
• W1753017813 countsByYear W17530178132022 @default.
• W1753017813 crossrefType "journal-article" @default.
• W1753017813 hasAuthorship W1753017813A5009018699 @default.
• W1753017813 hasAuthorship W1753017813A5020871813 @default.
• W1753017813 hasAuthorship W1753017813A5038015992 @default.
• W1753017813 hasAuthorship W1753017813A5078107525 @default.
• W1753017813 hasConcept C101762097 @default.
• W1753017813 hasConcept C102230213 @default.

• next