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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1967958045> ?p ?o ?g. }

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• W1967958045 abstract "We purified the activated recombinant glucicorticoid receptor (GR) overexpressed in insect cells by sequential chromatographies using Mono Q and Mono S columns. This procedure was based upon a new finding that the activated GR binds both to a Mono Q column and to a Mono S column at the same pH (pH 8.4). The entire chromatographies took about 3 h and GR represented 97% of the purified protein sample. The purified GR was able to bind specifically to a DNA fragment containing the glucocorticoid response element. This purification protocol will be applicable to the purification of native GR, point-mutated recombinant GR and other nuclear receptors." @default.
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• W1967958045 date "2001-12-01" @default.
• W1967958045 modified "2023-09-23" @default.
• W1967958045 title "Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system" @default.
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• W1967958045 doi "https://doi.org/10.1016/s0378-4347(01)00408-x" @default.
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