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• W1971728123 endingPage "569" @default.
• W1971728123 startingPage "553" @default.
• W1971728123 abstract "Neurofilaments accumulated in perikarya and dendrites of anterior horn cells and Purkinje cells of rabbit treated by aluminum chloride were analysed with a variety of techniques. Four different monoclonal antibodies against phosphorylated and nonphosphorylated epitopes on neurofilament H subunit were used to compare phosphorylation state of these accumulated neurofilaments with that of axonal neurofilaments. Although immunoblotting revealed no significant difference in phosphorylation between control and aluminum-treated brains, accumulated neurofilaments were immunocytochemically more phosphorylated than control perikaryal or dendritic neurofilaments. With detailed analysis of cryothin-section immunogold labeling, accumulated neurofilaments were, however, significantly less phosphorylated than axonal neurofilaments. With quick-freeze deep etching, core filaments of accumulated neurofilaments are as dense as axonal neurofilaments but much less regularly aligned. Cross-bridges of accumulated neurofilaments were less frequent and more branched than those of axonal neurofilaments, and when examined with combined immunocytochemistry and deep etching, were less phosphorylated. These results suggest that there is a relationship between the phosphorylation and the structural organization of neurofilaments. The phosphorylation of neurofilament H subunit may be necessary for formation of frequent and straight cross-bridges and resulting regular alignment of core filaments." @default.
• W1971728123 created "2016-06-24" @default.
• W1971728123 creator A5003214001 @default.
• W1971728123 creator A5048570680 @default.
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• W1971728123 date "1995-01-01" @default.
• W1971728123 modified "2023-09-25" @default.
• W1971728123 title "The organization of neurofilaments accumulated in perikaryon following aluminum administration: Relationship between structure and phosphorylation of neurofilaments" @default.
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• W1971728123 doi "https://doi.org/10.1016/0306-4522(94)00394-k" @default.
• W1971728123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7700539" @default.
• W1971728123 hasPublicationYear "1995" @default.
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